Rabbit Polyclonal Synapsin I phospho S9 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SYN1 phospho S9.
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- Frontiers in neuroscience 16:9054032022Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuJuan Fan,Li Li,LiRong Liu,He Li,XiaoHui Xian,WenBin LiPubMed 35860293
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SYN1 phospho S9. The exact immunogen used to generate this antibody is proprietary information. Database link P17600
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Expected | Tested |
| Rat | Tested | Expected |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed:21441247, PubMed:23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).
Alternative names
Synapsin-1, Brain protein 4.1, Synapsin I, SYN1
Recommended products
Rabbit Polyclonal Synapsin I phospho S9 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SYN1 phospho S9.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SYN1 phospho S9. The exact immunogen used to generate this antibody is proprietary information. Database link P17600
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is specific for endogenous levels of the ~78 kDa synapsin I doublet protein phosphorylated at Ser . Also weakly labels the ~55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser on synapsin I. Immunolabeling is completely eliminated by treatment with λ- Ptase.
- Concentration
- Purification notes
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.
- Biological function summary
Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.
- Pathways
Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.
- Associated diseases and disorders
Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Synapsin I (phospho S9) antibody (ab254033)
All lanes: Western blot - Anti-Synapsin I (phospho S9) antibody (ab254033) at 1/1000 dilution
Lane 1: Un-treated rat cortical lysate
Lane 2: Rat cortical lysate, treated with lambda phosphatase, 1200 units for 30 mins
Predicted band size: 74 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I (phospho S9) antibody (ab254033)
Cultured mouse caudate neurons labeling Synapsin I (phospho S9) using ab254033 at a 1/500 dilution in ICC/IF.
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