AB254033

Anti-Synapsin I (phospho S9) antibody

Name: Anti-Synapsin I (phospho S9) antibody (ab254033) | Abcam
Brand: Abcam Limited
SKU: AB254033
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Synapsin I phospho S9 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SYN1 phospho S9.

View Alternative Names

Synapsin-1, Brain protein 4.1, Synapsin I, SYN1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I (phospho S9) antibody (AB254033)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I (phospho S9) antibody (AB254033)

Cultured mouse caudate neurons labeling Synapsin I (phospho S9) using ab254033 at a 1/500 dilution in ICC/IF.

Supplier Data

Western blot - Anti-Synapsin I (phospho S9) antibody (AB254033)

All lanes:

Western blot - Anti-Synapsin I (phospho S9) antibody (ab254033) at 1/1000 dilution

Lane 1:

Un-treated rat cortical lysate

Lane 2:

Rat cortical lysate, treated with lambda phosphatase, 1200 units for 30 mins

Predicted band size: 74 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SYN1 phospho S9. The exact immunogen used to generate this antibody is proprietary information.

P17600

Specificity

This antibody is specific for endogenous levels of the ~78 kDa synapsin I doublet protein phosphorylated at Ser . Also weakly labels the ~55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser on synapsin I. Immunolabeling is completely eliminated by treatment with λ- Ptase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Storage buffer

pH: 7.5 Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.

Biological function summary

Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.

Pathways

Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.

Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed : 21441247, PubMed : 23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).

See full target information SYN1 phospho S9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 16:905403 PubMed35860293

2022

Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

ShuJuan Fan,Li Li,LiRong Liu,He Li,XiaoHui Xian,WenBin Li

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

View all publications

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Product promise

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Anti-Synapsin I (phospho S9) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I (phospho S9) antibody (AB254033)

Western blot - Anti-Synapsin I (phospho S9) antibody (AB254033)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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