AB76260

Anti-Synapsin I (phospho S9) antibody [EP2162Y]

20ul selling size
RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal Synapsin I phospho S9 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

Synapsin-1, Brain protein 4.1, Synapsin I, SYN1

6 Images

Unknown

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

All lanes:

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

Lane 1:

Human brain tissue lysate, untreated at 10 µg

Lane 2:

Human brain tissue lysate, treated with AP at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 74 kDa

Observed band size: 74 kDa

false

Lab

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/5000 dilution

Lane 1:

Human brain whole cell lysate - untreated at 10 µg

Lane 2:

Human brain whole cell lysate - treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Lab

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Exposure time :

Lanes 1 and 2 : 1 second.

Lanes 3 and 4 : 15 seconds.

Blocking and dilution buffer :

Lanes 1 and 2 : 5% NFDM/TBST.

Lanes 3 and 4 : 2% BSA/TBST.

All lanes:

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution

Lanes 1 and 3:

Human brain whole cell lysate - untreated at 10 µg

Lanes 2 and 4:

Human brain whole cell lysate - treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Lab

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/200 dilution

Lane 1:

Mouse brain whole cell lysate - untreated at 10 µg

Lane 2:

Mouse brain whole cell lysate - treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Blocking and dilution buffer : 2% BSA/TBST.

All lanes:

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution

Lane 1:

Rat brain whole cell lysate - untreated at 10 µg

Lane 2:

Rat brain whole cell lysate - treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Exposure time: 1s

Unknown

Dot Blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Dot blot analysis of Synapsin I (pS9) phospho peptide (lane 1) and Synapsin I non-phospho peptide (lane 2) labelling Synapsin I (pS9) with ab76260 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2162Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.

Biological function summary

Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.

Pathways

Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.

Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed : 21441247, PubMed : 23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).

See full target information SYN1 pS9

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 19:1618-1624 PubMed38051907

2023

Melatonin improves synapse development by PI3K/Akt signaling in a mouse model of autism spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Luyi Wang,Man Xu,Yan Wang,Feifei Wang,Jing Deng,Xiaoya Wang,Yu Zhao,Ailing Liao,Feng Yang,Shali Wang,Yingbo Li

Brain and behavior 13:e3075 PubMed37226399

2023

Effects of chronic sleep restriction on the neuro-phenotypes of Ctnnd2 knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Man Xu,Xiaoya Wang,Luyi Wang,Shali Wang,Jing Deng,Yan Wang,Yingbo Li,Sen Pan,Ailing Liao,Yihao Tao,Shujiang Tan

Cell death & disease 13:786 PubMed36097267

2022

Crosstalk of Synapsin1 palmitoylation and phosphorylation controls the dynamicity of synaptic vesicles in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Yan,Huicong Liu,Tao Zhou,Pu Sun,Yilin Wang,Xibin Wang,Lin Zhang,Tian Wang,Jing Dong,Jiangli Zhu,Luxian Lv,Wenqiang Li,Shiqian Qi,Yinming Liang,Eryan Kong

Theranostics 12:3656-3675 PubMed35664081

2022

Requirement of hippocampal DG nNOS-CAPON dissociation for the anxiolytic and antidepressant effects of fluoxetine.

Applications

Unspecified application

Species

Unspecified reactive species

Hu-Jiang Shi,Dan-Lian Wu,Rong Chen,Na Li,Li-Juan Zhu

Journal of nanobiotechnology 19:324 PubMed34654438

2021

Neurotransmitter-stimulated neuron-derived sEVs have opposite effects on amyloid β-induced neuronal damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxiao Dou,Junchao Xie,Yan Tan,Min Zhang,Yanxin Zhao,Xueyuan Liu

American journal of physiology. Regulatory, integrative and comparative physiology 308:R1-9 PubMed25394828

2014

NMDA-type glutamate receptors participate in reduction of food intake following hindbrain melanocortin receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos A Campos,Robert C Ritter

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:12636-45 PubMed25232103

2014

Central vagal afferent endings mediate reduction of food intake by melanocortin-3/4 receptor agonist.

Applications

IHC-FoFr

Species

Rat

Carlos A Campos,Hiroko Shiina,Robert C Ritter

Molecular and cellular biology 33:1442-55 PubMed23382070

2013

Activation of EphA receptors mediates the recruitment of the adaptor protein Slap, contributing to the downregulation of N-methyl-D-aspartate receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia Semerdjieva,Hayder H Abdul-Razak,Sharifah S Salim,Rafael J Yáñez-Muñoz,Philip E Chen,Victor Tarabykin,Pavlos Alifragis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synapsin I (phospho S9) antibody [EP2162Y]

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

Dot Blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (AB76260)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise