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Rabbit Recombinant Monoclonal Synaptogyrin 3 antibody. Suitable for ICC/IF, IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-FrIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Expected
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Tested
Tested
Tested
Tested

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/50

Notes

-

Species

Mouse

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    May play a role in regulated exocytosis. May indirectly regulate the activity of the plasma membrane dopamine transporter SLC6A3 and thereby regulate dopamine transport back from the synaptic cleft into the presynaptic terminal.

    Alternative names

    Synaptogyrin-3, SYNGR3

    Recommended products

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    Rabbit Recombinant Monoclonal Synaptogyrin 3 antibody. Suitable for ICC/IF, IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

    Alternative names

    Synaptogyrin-3, SYNGR3

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR25082-15

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Supplementary info

    Activity summary

    Synaptogyrin 3 also known as SYNGR3 functions as a component of vesicular transport within the synaptic terminals. With a molecular mass of approximately 25 kDa this protein localizes mainly in the brain prominently within the presynaptic nerve terminals. It plays a role in neurotransmitter release by being involved in modulating synaptic vesicle exocytosis. SYNGR3 shares structural characteristics with other members of the synaptogyrin family marked by its transmembrane domains.

    Biological function summary

    SYNGR3 influences synaptic plasticity and neurotransmission efficiency. This protein acts in conjunction with synaptophysin part of a larger complex that regulates vesicle biogenesis and release dynamics. Its presence contributes to sustaining long-term potentiation and synaptic integrity rendering it important for memory and learning processes. Its activity is essential in maintaining effective communication between neurons across the synapses.

    Pathways

    SYNGR3 plays a significant role in the synaptic vesicle cycle pathway. This pathway involves recycling and mobilization of synaptic vesicles interacting with proteins such as synaptotagmin and VAMP2. Another key interaction involves the SNARE complex which is important for vesicle fusion and subsequent neurotransmitter release. SYNGR3 aids in aligning vesicles for efficient fusion impacting neuronal signaling pathways.

    Associated diseases and disorders

    Dysfunction in SYNGR3 relates to neurological conditions such as schizophrenia and Alzheimer's disease. Abnormalities in SYNGR3 expression or function may disrupt synaptic transmission contributing to cognitive deficits seen in these disorders. There is also a connection between SYNGR3 and amyloid precursor protein (APP) suggesting disruptions in their interactions might exacerbate neurodegenerative processes. Understanding SYNGR3's role may provide insight into potential therapeutic targets for these conditions.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    11 product images

    • Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Synaptogyrin 3 with ab302613 at 1/500 (1.008 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p>

      .

    • Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Synaptogyrin 3 with ab302613 at 1/500 (1.008 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p>

      .

    • Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Synaptogyrin 3 with ab302613 at 1/50 (10.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing synaptic staining in rat primary neural/glia cell.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
      Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Synaptogyrin 3 with ab302613 at 1/50 (10.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing synaptic staining in mouse primary neural/glia cell.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection.Negative control: C2C12 is observed. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
      Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining on mouse pancreas. The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse hippocampus (PMID: 33472038). The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Negative controls: 293T (PMID: 14755516), C2C12
      Exposure time: Lane 1: 48 seconds
      Lane 2, 3 and 4: 136 seconds

      All lanes: Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613) at 1/1000 dilution

      Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 50 µg

      Lane 2: C2C12 (mouse myoblasts myoblast), whole cell lysate 50 µg

      Lane 3: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 50 µg

      Lane 4: 293T (human embryonic kidney epithelial cell), whole cell lysate 50 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

      Observed band size: 26 kDa

    • Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Negative control: pancreas (Database :HPA, the human protein atlas)
      Exposure time: 1 seconds

      All lanes: Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613) at 1/1000 dilution

      Lane 1: Rat hypothalamus tissue lysate 50 µg

      Lane 2: Rat pancreas tissue lysate 50 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

      Observed band size: 26 kDa

      Exposure time: 1s

    • Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Negative control: pancreas (Database :HPA, the human protein atlas)
      Exposure time: 1 seconds

      All lanes: Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613) at 1/1000 dilution

      Lane 1: Mouse brain tissue lysate 50 µg

      Lane 2: Mouse hypothalamus tissue lysate 50 µg

      Lane 3: Mouse pancreas tissue lysate 50 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

      Observed band size: 26 kDa

      Exposure time: 1s

    • Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613), expandable thumbnail

      Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Negative control: pancreas (Database :HPA, the human protein atlas)
      Exposure time: Lane 1 and 3: 48 seconds
      Lane 2: 5.5 seconds

      All lanes: Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613) at 1/1000 dilution

      Lane 1: Human eyeball tissue lysate 50 µg

      Lane 2: Human hypothalamus tissue lysate 50 µg

      Lane 3: Human pancreas tissue lysate 50 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

      Observed band size: 26 kDa

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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