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AB302613

Anti-Synaptogyrin 3 antibody [EPR25082-15]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
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Rabbit Recombinant Monoclonal Synaptogyrin 3 antibody. Suitable for ICC/IF, IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

View Alternative Names

Synaptogyrin-3, SYNGR3

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on mouse pancreas. The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse hippocampus (PMID : 33472038). The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Synaptogyrin 3 with ab302613 at 1/500 (1.008 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Synaptogyrin 3 with ab302613 at 1/500 (1.008 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Synaptogyrin 3 with ab302613 at 1/50 (10.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing synaptic staining in mouse primary neural/glia cell.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.Negative control : C2C12 is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Synaptogyrin 3 with ab302613 at 1/50 (10.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing synaptic staining in rat primary neural/glia cell.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative controls : 293T (PMID : 14755516), C2C12 Exposure time : Lane 1 : 48 seconds Lane 2, 3 and 4 : 136 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 50 µg

Lane 2:

C2C12 (mouse myoblasts myoblast), whole cell lysate 50 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 50 µg

Lane 4:

293T (human embryonic kidney epithelial cell), whole cell lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : pancreas (Database : HPA, the human protein atlas) Exposure time : Lane 1 and 3 : 48 seconds Lane 2 : 5.5 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613) at 1/1000 dilution

Lane 1:

Human eyeball tissue lysate 50 µg

Lane 2:

Human hypothalamus tissue lysate 50 µg

Lane 3:

Human pancreas tissue lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : pancreas (Database : HPA, the human protein atlas) Exposure time : 1 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate 50 µg

Lane 2:

Mouse hypothalamus tissue lysate 50 µg

Lane 3:

Mouse pancreas tissue lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

Exposure time: 1s

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)
  • WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (AB302613)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : pancreas (Database : HPA, the human protein atlas) Exposure time : 1 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (ab302613) at 1/1000 dilution

Lane 1:

Rat hypothalamus tissue lysate 50 µg

Lane 2:

Rat pancreas tissue lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

Exposure time: 1s

  • Carrier free

    Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25082-15

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-Fr, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptogyrin 3 also known as SYNGR3 functions as a component of vesicular transport within the synaptic terminals. With a molecular mass of approximately 25 kDa this protein localizes mainly in the brain prominently within the presynaptic nerve terminals. It plays a role in neurotransmitter release by being involved in modulating synaptic vesicle exocytosis. SYNGR3 shares structural characteristics with other members of the synaptogyrin family marked by its transmembrane domains.
Biological function summary

SYNGR3 influences synaptic plasticity and neurotransmission efficiency. This protein acts in conjunction with synaptophysin part of a larger complex that regulates vesicle biogenesis and release dynamics. Its presence contributes to sustaining long-term potentiation and synaptic integrity rendering it important for memory and learning processes. Its activity is essential in maintaining effective communication between neurons across the synapses.

Pathways

SYNGR3 plays a significant role in the synaptic vesicle cycle pathway. This pathway involves recycling and mobilization of synaptic vesicles interacting with proteins such as synaptotagmin and VAMP2. Another key interaction involves the SNARE complex which is important for vesicle fusion and subsequent neurotransmitter release. SYNGR3 aids in aligning vesicles for efficient fusion impacting neuronal signaling pathways.

Dysfunction in SYNGR3 relates to neurological conditions such as schizophrenia and Alzheimer's disease. Abnormalities in SYNGR3 expression or function may disrupt synaptic transmission contributing to cognitive deficits seen in these disorders. There is also a connection between SYNGR3 and amyloid precursor protein (APP) suggesting disruptions in their interactions might exacerbate neurodegenerative processes. Understanding SYNGR3's role may provide insight into potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in regulated exocytosis. May indirectly regulate the activity of the plasma membrane dopamine transporter SLC6A3 and thereby regulate dopamine transport back from the synaptic cleft into the presynaptic terminal.
See full target information SYNGR3
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Product promise

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For full details, please see our Terms & Conditions

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