Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on mouse pancreas. The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Synaptogyrin 3 with ab302613 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse hippocampus (PMID : 33472038). The section was incubated with ab302613 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613 the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Synaptogyrin 3 with ab302613 at 1/500 (1.008 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613 the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Synaptogyrin 3 with ab302613 at 1/500 (1.008 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Synaptogyrin 3 with ab302613 at 1/50 (10.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing synaptic staining in mouse primary neural/glia cell.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.Negative control : C2C12 is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Synaptogyrin 3 with ab302613 at 1/50 (10.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing synaptic staining in rat primary neural/glia cell.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

• WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative controls : 293T (PMID : 14755516), C2C12 Exposure time : Lane 1 : 48 seconds Lane 2, 3 and 4 : 136 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (<a href='/en-us/products/primary-antibodies/synaptogyrin-3-antibody-epr25082-15-ab302613'>ab302613</a>) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 50 µg

Lane 2:

C2C12 (mouse myoblasts myoblast), whole cell lysate 50 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 50 µg

Lane 4:

293T (human embryonic kidney epithelial cell), whole cell lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : pancreas (Database : HPA, the human protein atlas) Exposure time : Lane 1 and 3 : 48 seconds Lane 2 : 5.5 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (<a href='/en-us/products/primary-antibodies/synaptogyrin-3-antibody-epr25082-15-ab302613'>ab302613</a>) at 1/1000 dilution

Lane 1:

Human eyeball tissue lysate 50 µg

Lane 2:

Human hypothalamus tissue lysate 50 µg

Lane 3:

Human pancreas tissue lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : pancreas (Database : HPA, the human protein atlas) Exposure time : 1 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (<a href='/en-us/products/primary-antibodies/synaptogyrin-3-antibody-epr25082-15-ab302613'>ab302613</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate 50 µg

Lane 2:

Mouse hypothalamus tissue lysate 50 µg

Lane 3:

Mouse pancreas tissue lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] - BSA and Azide free (AB302614)

This data was developed using ab302613, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : pancreas (Database : HPA, the human protein atlas) Exposure time : 1 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-15] (<a href='/en-us/products/primary-antibodies/synaptogyrin-3-antibody-epr25082-15-ab302613'>ab302613</a>) at 1/1000 dilution

Lane 1:

Rat hypothalamus tissue lysate 50 µg

Lane 2:

Rat pancreas tissue lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

Exposure time: 1s