Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)

This data was developed using ab302704, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Synaptogyrin 3 with ab302704 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on acinar cells in human pancreas (PMID : 9760194). The section was incubated with ab302704 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)

This data was developed using ab302704, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human hypothalamus tissue labeling Synaptogyrin 3 with ab302704 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human hypothalamus. The section was incubated with ab302704 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)

This data was developed using ab302704, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Synaptogyrin 3 with ab302704 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human placenta (PMID : 9760194). The section was incubated with ab302704 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)

This data was developed using ab302704, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Synaptogyrin 3 with ab302704 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebrum (PMID : 9760194). The section was incubated with ab302704 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)

This data was developed using ab302704, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 103 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (<a href='/en-us/products/primary-antibodies/synaptogyrin-3-antibody-epr25082-29-ab302704'>ab302704</a>) at 1/1000 dilution

All lanes:

Human hypothalamus tissue lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

Exposure time: 103s

• WB

Supplier Data

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)

This data was developed using ab302704, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : pancreas (Database : HPA, the human protein atlas) Exposure time : 37 seconds

All lanes:

Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (<a href='/en-us/products/primary-antibodies/synaptogyrin-3-antibody-epr25082-29-ab302704'>ab302704</a>) at 1/1000 dilution

Lane 1:

Human eyeball tissue lysate 50 µg

Lane 2:

Human pancreas tissue lysate 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 26 kDa

false

Exposure time: 37s