Rabbit Recombinant Monoclonal Synaptogyrin 3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--western-blot-img401150.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)")
![Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--western-blot-img401151.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401183.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401185.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (AB302705)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Target data
Function
May play a role in regulated exocytosis. May indirectly regulate the activity of the plasma membrane dopamine transporter SLC6A3 and thereby regulate dopamine transport back from the synaptic cleft into the presynaptic terminal.
Alternative names
Synaptogyrin-3, SYNGR3
Recommended products
Rabbit Recombinant Monoclonal Synaptogyrin 3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25082-29
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synaptogyrin 3 also known as SYNGR3 functions as a component of vesicular transport within the synaptic terminals. With a molecular mass of approximately 25 kDa this protein localizes mainly in the brain prominently within the presynaptic nerve terminals. It plays a role in neurotransmitter release by being involved in modulating synaptic vesicle exocytosis. SYNGR3 shares structural characteristics with other members of the synaptogyrin family marked by its transmembrane domains.
- Biological function summary
SYNGR3 influences synaptic plasticity and neurotransmission efficiency. This protein acts in conjunction with synaptophysin part of a larger complex that regulates vesicle biogenesis and release dynamics. Its presence contributes to sustaining long-term potentiation and synaptic integrity rendering it important for memory and learning processes. Its activity is essential in maintaining effective communication between neurons across the synapses.
- Pathways
SYNGR3 plays a significant role in the synaptic vesicle cycle pathway. This pathway involves recycling and mobilization of synaptic vesicles interacting with proteins such as synaptotagmin and VAMP2. Another key interaction involves the SNARE complex which is important for vesicle fusion and subsequent neurotransmitter release. SYNGR3 aids in aligning vesicles for efficient fusion impacting neuronal signaling pathways.
- Associated diseases and disorders
Dysfunction in SYNGR3 relates to neurological conditions such as schizophrenia and Alzheimer's disease. Abnormalities in SYNGR3 expression or function may disrupt synaptic transmission contributing to cognitive deficits seen in these disorders. There is also a connection between SYNGR3 and amyloid precursor protein (APP) suggesting disruptions in their interactions might exacerbate neurodegenerative processes. Understanding SYNGR3's role may provide insight into potential therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--western-blot-img401150.jpg "Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)")
Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)
This data was developed using Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: pancreas (Database :HPA, the human protein atlas)
Exposure time: 37 secondsAll lanes: Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704) at 1/1000 dilution
Lane 1: Human eyeball tissue lysate 50 µg
Lane 2: Human pancreas tissue lysate 50 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution
Observed band size: 26 kDa
Exposure time: 37s
![Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--western-blot-img401151.jpg "Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)")
Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)
This data was developed using Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 103 secondsAll lanes: Western blot - Anti-Synaptogyrin 3 antibody [EPR25082-29] (Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704) at 1/1000 dilution
All lanes: Human hypothalamus tissue lysate 50 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution
Observed band size: 26 kDa
Exposure time: 103s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401183.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)
This data was developed using Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Synaptogyrin 3 with Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human cerebrum (PMID: 9760194). The section was incubated with Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401184.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)
This data was developed using Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hypothalamus tissue labeling Synaptogyrin 3 with Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human hypothalamus. The section was incubated with Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401185.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)
This data was developed using Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Synaptogyrin 3 with Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human placenta (PMID: 9760194). The section was incubated with Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401186.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)
This data was developed using Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Synaptogyrin 3 with Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704 at 1/2000 (0.246 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: No staining on acinar cells in human pancreas (PMID: 9760194). The section was incubated with Anti-Synaptogyrin 3 antibody [EPR25082-29] ab302704 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705), expandable thumbnail](https://content.abcam.com/products/images/synaptogyrin-3-antibody-epr25082-29-bsa-and-azide-free-ab302705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401186.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody [EPR25082-29] (BSA and Azide free) (ab302705)")
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