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Rabbit Multiclonal Synaptojanin antibody. Suitable for WB, IHC-Fr and reacts with Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SYNJ1.

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Images

Western blot - Anti-Synaptojanin 1 antibody [RP23040243] (AB308136), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Synaptojanin 1 antibody [RP23040243] (AB308136), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Recombinant Fragment Protein within Human SYNJ1. Database link O43426

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-Fr
Mouse
Tested
Tested
Rat
Tested
Expected

Tested
Tested

Species

Rat

Dilution info

2.5 µg/mL

Notes

-

Species

Mouse

Dilution info

2.5 µg/mL

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/250

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Phosphatase that acts on various phosphoinositides, including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate (PubMed:23804563, PubMed:27435091). Has a role in clathrin-mediated endocytosis (By similarity). Hydrolyzes PIP2 bound to actin regulatory proteins resulting in the rearrangement of actin filaments downstream of tyrosine kinase and ASH/GRB2 (By similarity).

Alternative names

Recommended products

Rabbit Multiclonal Synaptojanin antibody. Suitable for WB, IHC-Fr and reacts with Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SYNJ1.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Recombinant Fragment Protein within Human SYNJ1. Database link O43426
Clone number

RP23040243

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Synaptojanin 1 antibody [RP23040243] (ab308136), expandable thumbnail

    Western blot - Anti-Synaptojanin 1 antibody [RP23040243] (ab308136)

    Western blot analysis was performed on Membrane enriched extracts (30 µg lysate) of PC-12 (Lane 1), PC-12 treated with NGF and KCl (NGF at 200 nM, KCl at 50 nM for 7 days) (Lane 2) and tissue extracts of Mouse Brain (Lane 3). The blots were probed with Anti-Synaptojanin Recombinant Rabbit Polyclonal Antibody (ab308136, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.25 µg/mL, 1:4000 dilution). A 173 kDa band corresponding to Synaptojanin was observed specifically in NGF differentiated PC-12 and tissue tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

    Lane 1: Membrane enriched extracts of PC-12 cell lysate at 30 µg

    Lane 2: Membrane enriched extracts of PC-12 cell lysate treated with NGF and KCl (NGF at 200 nM, KCl at 50 nM for 7 days) at 30 µg

    Lane 3: Mouse Brain tissue lysates at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Developed using the ECL technique.

  • Immunohistochemistry (Frozen sections) - Anti-Synaptojanin 1 antibody [RP23040243] (ab308136), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Synaptojanin 1 antibody [RP23040243] (ab308136)

    Immunohistochemical analysis 4% PFA-fixed, 0.1% Triton-X 100-permeabilized mouse brain tissue labelling Synaptojainin 1 with ab308136 at 1/250 dilution, followed by a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000.
    Nuclei (blue) were stained with DAPI
    Panel (a) represents staining with the matched isotype control.
    Panel (b) shows a representative section stained for Synaptojainin 1 (green).
    The images were captured at 20X magnification.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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