Rabbit Polyclonal Synaptophysin antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 60 publications. Immunogen corresponding to Synthetic Peptide within Rat Syp aa 250-300.
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- International journal of molecular sciences 24:2023Synaptamide Ameliorates Hippocampal Neurodegeneration and Glial Activation in Mice with Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Tyrtyshnaia,Olga Manzhulo,Igor ManzhuloPubMed 37373162
- iScience 26:1067442023Multi-omics analysis of a drug-induced model of bipolar disorder in zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYameng Li,Lin Zhang,Mingcai Mao,Linjuan He,Tiancai Wang,Yecan Pan,Xiaoyu Zhao,Zishu Li,Xiyan Mu,Yongzhong Qian,Jing QiuPubMed 37207274
- Neural regeneration research 18:1090-10982022Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Guo,Yuan-Yuan Wang,Ting-Ting Sun,Jia-Jia Xu,Pan Yang,Cai-Yun Ma,Wei-Jun Guan,Chun-Jing Wang,Gao-Feng Liu,Chang-Qing LiuPubMed 36254998
- Disease models & mechanisms 15:2022Bisphenol-A impairs synaptic formation and function by RGS4-mediated regulation of BDNF signaling in the cerebral cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung-Ae Hyun,Moon Yi Ko,Sumi Jang,Byoung-Seok Lee,Jaerang Rho,Kee K Kim,Woo-Yang Kim,Minhan KaPubMed 35781563
- Theranostics 12:5389-54032022Subpial delivery of adeno-associated virus 9-synapsin-caveolin-1 () preserves motor neuron and neuromuscular junction morphology, motor function, delays disease onset, and extends survival in hSOD1 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Wang,Taiga Ichinomiya,Paul Savchenko,Dongsheng Wang,Atsushi Sawada,Xiaojing Li,Tiffany Duong,Wenxi Li,Jacqueline A Bonds,Eun Jung Kim,Atsushi Miyanohara,David M Roth,Hemal H Patel,Piyush M Patel,Takahiro Tadokoro,Martin Marsala,Brian P HeadPubMed 35910808
- Communications biology 5:6722022KHSRP loss increases neuronal growth and synaptic transmission and alters memory consolidation through RNA stabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah L Olguin,Priyanka Patel,Courtney N Buchanan,Michela Dell'Orco,Amy S Gardiner,Robert Cole,Lauren S Vaughn,Anitha Sundararajan,Joann Mudge,Andrea M Allan,Pavel Ortinski,Jonathan L Brigman,Jeffery L Twiss,Nora I Perrone-BizzozeroPubMed 35798971
- Anesthesiology 137:212-2312022Isoflurane Disrupts Postsynaptic Density-95 Protein Interactions Causing Neuronal Synapse Loss and Cognitive Impairment in Juvenile Mice via Canonical NO-mediated Protein Kinase-G Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSwati Agarwal,Michele L Schaefer,Caroline Krall,Roger A JohnsPubMed 35504002
- The Journal of neuroscience : the official journal of the Society for Neuroscience 42:3066-30792022Extrasynaptic NMDA Receptors Bidirectionally Modulate Intrinsic Excitability of Inhibitory Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLulu Yao,Yi Rong,Xiaoyan Ma,Haifu Li,Di Deng,Yongjun Chen,Sungchil Yang,Tao Peng,Tao Ye,Feixue Liang,Nenggui Xu,Qiang ZhouPubMed 35197319
- Frontiers in aging neuroscience 14:8145602022Low-Intensity Focused Ultrasound Stimulation Ameliorates Working Memory Dysfunctions in Vascular Dementia Rats Improving Neuronal Environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFaqi Wang,Qian Wang,Ling Wang,Jing Ren,Xizi Song,Yutao Tian,Chenguang Zheng,Jiajia Yang,Dong MingPubMed 35264943
- Autism research : official journal of the International Society for Autism Research 15:791-8052022Cerebellar demyelination and neurodegeneration associated with mTORC1 hyperactivity may contribute to the developmental onset of autism-like neurobehavioral phenotype in a rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesViera Kútna,Valerie Bríd O'Leary,Cyril Hoschl,Saak V OvsepianPubMed 35178882
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Syp aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link P07825
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Predicted | Predicted |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Alternative names
Synaptophysin, Major synaptic vesicle protein p38, Syp
Recommended products
Rabbit Polyclonal Synaptophysin antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 60 publications. Immunogen corresponding to Synthetic Peptide within Rat Syp aa 250-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Syp aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link P07825
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.
- Biological function summary
Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.
- Pathways
Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.
- Associated diseases and disorders
Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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9 product images
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
Western blot - Anti-Synaptophysin antibody (ab32594)
Western blot analysis was performed on tissue extracts (30 μg lysate). The blot was probed with ab32594 (1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A 34 kDa band corresponding to Synaptophysin was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be Synaptophysin negative.
All lanes: Western blot - Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 30 µg
Lane 2: Rat brain tissue lysate at 30 µg
Lane 3: Rat liver tissue lysate at 30 µg
Lane 4: Rat kidney tissue lysate at 30 µg
Predicted band size: 33 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Synaptophysin antibody (ab32594)
All lanes: Western blot - Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution
All lanes: Apteronotus leptorhynchus brain tissue lysate at 50 µg
SecondaryAll lanes: AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa, 35 kDa
Exposure time: 7min
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence in rat hippocampal neurons. The cells were fixed with 1% formaldehyde in fume hood at room temperature for 10 minutes.
ab32594 is used at 1:1500 dilution and DyLight 649 Conjugated Goat anti-Rabbit IgG used as secondary antibody at 1:1500 dilution.
Western blot - Anti-Synaptophysin antibody (ab32594)
All lanes: Western blot - Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution
All lanes: Rat brain
Predicted band size: 33 kDa
Observed band size: 33.8 kDa
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