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Rabbit Recombinant Monoclonal Synaptophysin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 59 publications.


Images

Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (AB52636), expandable thumbnail
  • Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (AB52636), expandable thumbnail
  • Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (AB52636), expandable thumbnail
  • Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (AB52636), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (AB52636), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Expected
Expected
Mouse
Not recommended
Tested
Expected
Expected
Rat
Not recommended
Tested
Tested
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/250 - 1/500
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/20 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Alternative names

Synaptophysin, Major synaptic vesicle protein p38, SYP

Recommended products

Rabbit Recombinant Monoclonal Synaptophysin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 59 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1098Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Associated diseases and disorders

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636)

    Synaptophysin Western blot staining using rabbit Anti-Synaptophysin antibody

    Lanes 1-3: Merged signal (red and green). Green - ab52636 observed at 38 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab52636 Anti-Synaptophysin antibody [EP1098Y] was shown to specifically react with Synaptophysin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human SYP (Synaptophysin) knockout HEK-293T cell line ab267272 (knockout cell lysate Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab257060) was used. Wild-type and Synaptophysin knockout samples were subjected to SDS-PAGE. ab52636 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: SYP knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell line (Human SYP (Synaptophysin) knockout HEK-293T cell line ab267272)

    Lane 3: Human brain tissue lysate

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636)

    Synaptophysin Western blot staining using rabbit Anti-Synaptophysin antibody

    Lanes 1-3: Merged signal (red and green). Green - ab52636 observed at 38 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab52636 Anti-Synaptophysin antibody [EP1098Y] was shown to specifically react with Synaptophysin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human SYP (Synaptophysin) knockout HEK-293T cell line ab267272 (knockout cell lysate Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab257060) was used. Wild-type and Synaptophysin knockout samples were subjected to SDS-PAGE. ab52636 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: Syp knockout HEK293T cell lysate at 20 µg

    Lane 3: Human brain tissue lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636)

    Synaptophysin Western blot staining using rabbit Anti-Synaptophysin antibody

    Blocking/Diluting buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636) at 0.203 µg/mL

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat brain lysate tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636)

    Synaptophysin Western blot staining of Rat brain membrane using rabbit Anti-Synaptophysin antibody

    All lanes: Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636) at 1/200000 dilution

    All lanes: Rat brain membrane

    Secondary

    All lanes: Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636)

    Synaptophysin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Synaptophysin antibody

    Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Synaptophysin with ab52636 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining in PC-12 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636)

    Synaptophysin Flow Cytometry (Intracellular) staining using rabbit Anti-Synaptophysin antibody

    Overlay histogram showing PC12 cells stained with ab52636 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52636, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (ab52636)

    Synaptophysin Western blot staining using rabbit Anti-Synaptophysin antibody

    Synaptophysin western blot using anti-Synaptophysin antibody [EP1098Y] ab52636. Publication image and figure legend from Zhou, J., Liu, Z., et al., 2015, Int J Neuropsychopharmacol, PubMed 26364272.


    ab52636 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab52636 please see the product overview.

    Western-blot analysis of the 4 fractions (Hom, Syn, Ves, and synaptic junction) obtained by biochemical fractionation. The fractions were analyzed using antibodies against synaptophysin (synaptic vesicle marker), Stx1a (synaptic active zone marker), and PSD95 (postsynaptic density marker).

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