AB178412

Anti-Synaptophysin antibody [EPR1097-2] - Synaptic Marker

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Synaptophysin antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 4 publications.

View Alternative Names

Synaptophysin, Major synaptic vesicle protein p38, SYP

2 Images

Lab

Western blot - Anti-Synaptophysin antibody [EPR1097-2] - Synaptic Marker (AB178412)

All lanes:

Western blot - Anti-Synaptophysin antibody [EPR1097-2] - Synaptic Marker (ab178412) at 1/1000 dilution

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 38 kDa

false

Lab

Western blot - Anti-Synaptophysin antibody [EPR1097-2] - Synaptic Marker (AB178412)

All lanes:

Western blot - Anti-Synaptophysin antibody [EPR1097-2] - Synaptic Marker (ab178412) at 1/1000 dilution

All lanes:

Rat brain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 38 kDa

false

Carrier free

Anti-Synaptophysin antibody [EPR1097-2] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1097-2

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

See full target information SYP

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed39851539

2025

miR-223 and Chromogranin A Affect Inflammatory Immune Cell Activation in Liver Metastasis of Neuroendocrine Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Geisler,Katharina Detjen,Teresa Hellberg,Marlene Kohlhepp,Carsten Grötzinger,Jana Knorr,Ines Eichhorn,Raphael Mohr,Theresa Holtmann,Bertram Wiedenmann,Frank Tacke,Christoph Roderburg,Alexander Wree

Frontiers in endocrinology 15:1331231 PubMed38694940

2024

Elevated sortilin expression discriminates functional from non-functional neuroendocrine tumors and enables therapeutic targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Bolduan,Alexandra Wetzel,Yvonne Giesecke,Ines Eichhorn,Natalia Alenina,Michael Bader,Thomas E Willnow,Bertram Wiedenmann,Michael Sigal

Nature communications 14:3025 PubMed37230989

2023

Establishment of gastrointestinal assembloids to study the interplay between epithelial crypts and their mesenchymal niche.

Applications

Unspecified application

Species

Unspecified reactive species

Manqiang Lin,Kimberly Hartl,Julian Heuberger,Giulia Beccaceci,Hilmar Berger,Hao Li,Lichao Liu,Stefanie Müllerke,Thomas Conrad,Felix Heymann,Andrew Woehler,Frank Tacke,Nikolaus Rajewsky,Michael Sigal

Evidence-based complementary and alternative medic 2020:9145458 PubMed32308720

2020

Sesamin Promotes Neurite Outgrowth under Insufficient Nerve Growth Factor Condition in PC12 Cells through ERK1/2 Pathway and SIRT1 Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Sasimol Udomruk,Chayanut Kaewmool,Thanyaluck Phitak,Peraphan Pothacharoen,Prachya Kongtawelert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synaptophysin antibody [EPR1097-2] - Synaptic Marker

Western blot - Anti-Synaptophysin antibody [EPR1097-2] - Synaptic Marker (AB178412)

Western blot - Anti-Synaptophysin antibody [EPR1097-2] - Synaptic Marker (AB178412)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise