Anti-Synaptophysin antibody [SP11] - Synaptic Marker

• ICC

Lab

Immunocytochemistry - Anti-Synaptophysin antibody [SP11] - Synaptic Marker (AB16659)

ab16659 staining Synaptophysin in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16659 at a 1/100 dilution and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Synaptophysin antibody [SP11] - Synaptic Marker (AB16659)

IHC image of Synaptophysin staining in a section of frozen normal human pancreas performed on a Leica BOND^TM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab16659, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SP11] - Synaptic Marker (AB16659)

Immunohistochemical staining of human neuroendocrine tumor with ab16659.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SP11] - Synaptic Marker (AB16659)

Immunohistochemical staining of human pancreas with ab16659.

• WB

Unknown

Western blot - Anti-Synaptophysin antibody [SP11] - Synaptic Marker (AB16659)

All lanes:

Western blot - Anti-Synaptophysin antibody [SP11] - Synaptic Marker (ab16659) at 1/500 dilution

All lanes:

lysate prepared from human brain

Predicted band size: 33 kDa

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