Skip to main content

Anti-Synaptophysin antibody [SY38] is a mouse monoclonal antibody that is used to detect Synaptophysin in Flow cytometry, ICC/IF, IHC-Fr, IHC-P, Western blot. Suitable for Cow, Hamster, Human, Mouse, Rat samples.

- Cited in over 220 publications
- Trusted since 2001


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049), expandable thumbnail
  • Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049), expandable thumbnail
  • Flow Cytometry - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (AB8049), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (AB8049), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.5% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human SYP. Database link P08247

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IFIHC-Fr
Human
Predicted
Predicted
Predicted
Expected
Expected
Mouse
Expected
Tested
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Expected
Expected
Cow
Predicted
Predicted
Predicted
Expected
Expected
Hamster
Predicted
Predicted
Predicted
Expected
Expected

Tested
Tested

Species
Rat
Dilution info
1/20
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human, Cow, Hamster
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human, Cow, Hamster
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human, Cow, Hamster
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Hamster, Human, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Rat, Hamster, Human, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Alternative names

Recommended products

Anti-Synaptophysin antibody [SY38] is a mouse monoclonal antibody that is used to detect Synaptophysin in Flow cytometry, ICC/IF, IHC-Fr, IHC-P, Western blot. Suitable for Cow, Hamster, Human, Mouse, Rat samples.

- Cited in over 220 publications
- Trusted since 2001

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Native Full Length Protein corresponding to Human SYP. Database link P08247
Clone number
SY38
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

Product Specifications
Anti-Synaptophysin antibody [SY38] (ab8049) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB in cow, hamster, human, mouse, rat samples.
Anti-Synaptophysin antibody [SY38] (ab8049) specifically detects Synaptophysin (UniProt ID: P20488; Molecular weight: 34kDa) and is sold in 500 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Synaptophysin antibody [SY38] (ab8049) has high sensitivity and specificity.
Anti-Synaptophysin antibody [SY38] (ab8049) has been cited over 228 times in peer reviewed journals and is trusted by the scientific community.
Anti-Synaptophysin antibody [SY38] (ab8049) has 15 independent reviews from customers.


Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049), expandable thumbnail
    This image is courtesy of a customer review provided by Carl Hobbs

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

    Synaptophysin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Synaptophysin antibody

    ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

  • Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

    Synaptophysin Western blot staining using mouse Anti-Synaptophysin antibody

    All lanes: Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049) at 1/500 dilution

    Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg

    Lane 2: Brain (Mouse) Tissue Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Flow Cytometry - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049), expandable thumbnail

    Flow Cytometry - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

    Synaptophysin Flow Cytometry staining using mouse Anti-Synaptophysin antibody

    Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049), expandable thumbnail
    This image is courtesy of a customer review provided by Carl Hobbs.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)

    ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

    Synaptophysin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Synaptophysin antibody

    The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine. The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,
    secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.

    These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com