Anti-Synaptophysin antibody [SY38] is a mouse monoclonal antibody that is used to detect Synaptophysin in Flow cytometry, ICC/IF, IHC-Fr, IHC-P, Western blot. Suitable for Cow, Hamster, Human, Mouse, Rat samples.
- Cited in over 220 publications
- Trusted since 2001
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.5% BSA
Flow Cyt | WB | IHC-P | ICC/IF | IHC-Fr | |
---|---|---|---|---|---|
Human | Predicted | Predicted | Predicted | Expected | Expected |
Mouse | Expected | Tested | Tested | Expected | Expected |
Rat | Tested | Expected | Expected | Expected | Expected |
Cow | Predicted | Predicted | Predicted | Expected | Expected |
Hamster | Predicted | Predicted | Predicted | Expected | Expected |
Species | Dilution info | Notes |
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Species Rat | Dilution info 1/20 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
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Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Human, Cow, Hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Cow, Hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Cow, Hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Hamster, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Hamster, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
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Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Synaptophysin, Major synaptic vesicle protein p38, SYP
Anti-Synaptophysin antibody [SY38] is a mouse monoclonal antibody that is used to detect Synaptophysin in Flow cytometry, ICC/IF, IHC-Fr, IHC-P, Western blot. Suitable for Cow, Hamster, Human, Mouse, Rat samples.
- Cited in over 220 publications
- Trusted since 2001
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.5% BSA
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Synaptophysin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Synaptophysin antibody
ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
Synaptophysin Western blot staining using mouse Anti-Synaptophysin antibody
All lanes: Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049) at 1/500 dilution
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Brain (Mouse) Tissue Lysate at 10 µg
All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Predicted band size: 33 kDa
Observed band size: 38 kDa
Synaptophysin Flow Cytometry staining using mouse Anti-Synaptophysin antibody
Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.
Synaptophysin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Synaptophysin antibody
The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine. The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,
secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.
These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.
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