Anti-Synaptophysin antibody [SY38] - Synaptic Marker

5 product images

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  This image is courtesy of a customer review provided by Carl Hobbs

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

  Synaptophysin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Synaptophysin antibody

  ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

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  Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

  Synaptophysin Western blot staining using mouse Anti-Synaptophysin antibody

  All lanes: Western blot - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049) at 1/500 dilution

  Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg

  Lane 2: Brain (Mouse) Tissue Lysate at 10 µg

  Secondary

  All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution

  Predicted band size: 33 kDa

  Observed band size: 38 kDa

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  Flow Cytometry - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

  Synaptophysin Flow Cytometry staining using mouse Anti-Synaptophysin antibody

  Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
  
  

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  This image is courtesy of a customer review provided by Carl Hobbs.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

  Synaptophysin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Synaptophysin antibody

  ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] - Synaptic Marker (ab8049)

  Synaptophysin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Synaptophysin antibody

  The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine. The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,
  secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.

  These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.