Rabbit Polyclonal Synaptophysin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 117 publications. Immunogen corresponding to Synthetic Peptide within Human SYP aa 1-100.
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- The British journal of nutrition 131:1105-11142023Tomato and lemon extracts synergistically improve cognitive function by increasing brain-derived neurotrophic factor levels in aged mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyeong-No Yoon,Yidan Cui,Qing-Ling Quan,Dong Hun Lee,Jang-Hee Oh,Jin Ho ChungPubMed 38016800
- Frontiers in pharmacology 14:12116632023Antidepressant-like effects of the and citalopram combination are associated with structural changes in dendritic spines of granule cells in the dentate gyrus of rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNelly-Maritza Vega-Rivera,María Eva González-Trujano,Alexandra Luna-Angula,Laura Sánchez-Chapul,Erika Estrada-CamarenaPubMed 37900157
- Current research in neurobiology 5:1001052023Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMira Seidel,Sandeep Rajkumar,Christina Steffke,Vivien Noeth,Shreya Agarwal,Kevin Roger,Joanna Lipecka,Albert Ludolph,Chiara Ida Guerrera,Tobias Boeckers,Alberto CatanesePubMed 37576491
- Acta neuropathologica 146:451-4752023Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmr Aly,Zsofia I Laszlo,Sandeep Rajkumar,Tugba Demir,Nicole Hindley,Douglas J Lamont,Johannes Lehmann,Mira Seidel,Daniel Sommer,Mirita Franz-Wachtel,Francesca Barletta,Simon Heumos,Stefan Czemmel,Edor Kabashi,Albert Ludolph,Tobias M Boeckers,Christopher M Henstridge,Alberto CatanesePubMed 37488208
- Investigative ophthalmology & visual science 64:12023TBK1 Knockdown Alleviates Axonal Transport Deficits in Retinal Ganglion Cells Via mTORC1 Activation in a Retinal Damage Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Ye,Yuanyuan Hu,Bowen Zhao,Qianxue Mou,Yueqi Ni,Jing Luo,Lu Li,Hong Zhang,Yin ZhaoPubMed 37395713
- Nature communications 14:37702023Schizophrenia-associated NRXN1 deletions induce developmental-timing- and cell-type-specific vulnerabilities in human brain organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca Sebastian,Kang Jin,Narciso Pavon,Ruby Bansal,Andrew Potter,Yoonjae Song,Juliana Babu,Rafael Gabriel,Yubing Sun,Bruce Aronow,ChangHui PakPubMed 37355690
- Frontiers in cellular neuroscience 17:11629662023Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Ju Kim,Min-Jung You,Soyoung Sung,Chan Rim,Min-Soo KwonPubMed 37396924
- BMC biology 21:1132023CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Woelfle,Dhruva Deshpande,Simone Feldengut,Heiko Braak,Kelly Del Tredici,Francesco Roselli,Karl Deisseroth,Jens Michaelis,Tobias M Boeckers,Michael SchönPubMed 37221592
- iScience 26:1066742023Mitochondrial Bcl-xL promotes brain synaptogenesis by controlling non-lethal caspase activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrang Thi Minh Nguyen,Rudy Gadet,Marine Lanfranchi,Romane A Lahaye,Sozerko Yandiev,Olivier Lohez,Ivan Mikaelian,Lea Jabbour,Ruth Rimokh,Julien Courchet,Frédéric Saudou,Nikolay Popgeorgiev,Germain GilletPubMed 37182099
- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:2002-20202023Epilepsy-Related CDKL5 Deficiency Slows Synaptic Vesicle Endocytosis in Central Nerve Terminals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristiana Kontaxi,Daniela Ivanova,Elizabeth C Davenport,Peter C Kind,Michael A CousinPubMed 36759195
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SYP aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P08247
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
Associated Products
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Target data
Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Alternative names
Synaptophysin, Major synaptic vesicle protein p38, SYP
Recommended products
Rabbit Polyclonal Synaptophysin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 117 publications. Immunogen corresponding to Synthetic Peptide within Human SYP aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SYP aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P08247
- Purification technique
- Affinity purification Immunogen
- Specificity
The antibody does not cross-react other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.
- Biological function summary
Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.
- Pathways
Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.
- Associated diseases and disorders
Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody (ab14692)
Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded mouse brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomWestern blot - Anti-Synaptophysin antibody - Synaptic Marker (ab14692)
Synaptophysin Western blot staining using rabbit Anti-Synaptophysin antibody
Synaptophysin antibody (ab14692) western blot on mouse forebrain extract (10μg protein/lane). The blot was exposed for 10 minutes to autoradiographic film. Ladder markers from 7-194 kDa.
Lane 1 = 1/500 ab14692
Lane 2 = 1/200 ab14692All lanes: Western blot - Anti-Synaptophysin antibody - Synaptic Marker (ab14692)
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody (ab14692)
Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded rat brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
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