AB14692

Anti-Synaptophysin antibody - Synaptic Marker

Name: Anti-Synaptophysin antibody - Synaptic Marker (ab14692) | Abcam
Brand: Abcam Limited
SKU: AB14692
Price: 500 USD
Availability: InStock
Rating: 5 (9 reviews)

(9 Reviews)

(141 Publications)

Anti-Synaptophysin antibody (ab14692) is a rabbit polyclonal antibody detecting Synaptophysin in Western Blot, IHC-P. Suitable for Mouse, Rat.

- Over 110 publications
- Trusted since 2004

View Alternative Names

Synaptophysin, Major synaptic vesicle protein p38, SYP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody - Synaptic Marker (AB14692)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody - Synaptic Marker (AB14692)

Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded mouse brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody - Synaptic Marker (AB14692)

Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded rat brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.

Collaborator

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (AB14692)

Synaptophysin antibody (ab14692) western blot on mouse forebrain extract (10μg protein/lane). The blot was exposed for 10 minutes to autoradiographic film. Ladder markers from 7-194 kDa.

Lane 1 = 1/500 ab14692
Lane 2 = 1/200 ab14692

All lanes:

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (ab14692)

Predicted band size: 33 kDa

false

This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SYP aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P08247

Specificity

The antibody does not cross-react other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Synaptophysin antibody (ab14692) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.

What is the molecular weight of Synaptophysin?
Anti-Synaptophysin (ab14692) specifically detects a band for Synaptophysin (UniProt: P08247) at a molecular weight of 38kDa.

Trusted by the scientific community
Anti-Synaptophysin (ab14692) was first used in a scientific publication in 2004 and has been cited over 110 times in peer-reviewed journals.

Reviewed by scientists
Anti-Synaptophysin (ab14692) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

See full target information SYP

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Publications (141)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0328847 PubMed40720390

2025

Memory improving effect of silkworm larva on insulin resistance related cognitive impairment model.

Applications

Unspecified application

Species

Unspecified reactive species

Joohyun Hwang,Junhyuk Choi,Seung-Yun Cha,In-Seo Lee,Ji Hye Yoon,Duk Jin Jung,Chang Wook Lee,Seong Ryul Kim,Ji Hae Lee,Byeongyeob Jeon,Ji-Ho Park,Sungho Maeng,Hyunwoo Park

Cell communication and signaling : CCS 23:196 PubMed40275260

2025

Neurodegenerative disease-associated microRNAs acting as signaling molecules modulate CNS neuron structure and viability.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Kumbol,Andranik Ivanov,Hugo McGurran,Jutta Schüler,Yuanyuan Zhai,Katarzyna Ludwik,Lukas Hinkelmann,Mariam Brehm,Christina Krüger,Judit Küchler,Thomas Wallach,Markus Höltje,Dieter Beule,Harald Stachelscheid,Seija Lehnardt

Scientific reports 15:5811 PubMed39962142

2025

Nano-scaffold containing functional motif of stromal cell-derived factor 1 enhances neural stem cell behavior and synaptogenesis in traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Amin Bayat Tork,Mohsen Saberifar,Hamed Joneidi Yekta,Mehrdad Hajinejad,Hassan Hosseini Ravandi,Ali Gorji,Sajad Sahab Negah

Pflugers Archiv : European journal of physiology 477:371-391 PubMed39836224

2025

Lateral hypothalamic area high-frequency deep brain stimulation rescues memory decline in aged rat: behavioral, molecular, and electrophysiological study.

Applications

Unspecified application

Species

Unspecified reactive species

Abdelaziz M Hussein,Ahmed F Abouelnaga,Walaa Obydah,Somaya Saad,Marwa Abass,Asmaa Yehia,Eman M Ibrahim,Ahmed T Ahmed,Osama A Abulseoud

Journal of cellular physiology 240:e31477 PubMed39513231

2024

Dynamic mRNA Stability Buffer Transcriptional Activation During Neuronal Differentiation and Is Regulated by SAMD4A.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhou,Sherif Rashad,Daisuke Ando,Yuki Kobayashi,Teiji Tominaga,Kuniyasu Niizuma

Molecular and clinical oncology 21:91 PubMed39421232

2024

Potential neuroendocrine differentiation in poorly differentiated colorectal adenocarcinoma: A hidden trait?

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Rong,Ikuma Kato,Naoki Okubo,Sho Tsuyuki,Eriko Katsuta,Noritoshi Kobayashi,Kazuya Nakagawa,Mayumi Ozawa,Jun Watanabe,Atsushi Ishibe,Shoji Yamanaka,Satoshi Fujii,Itaru Endo,Yasushi Ichikawa

Molecules (Basel, Switzerland) 29: PubMed39407543

2024

The Expression of Cannabinoid and Cannabinoid-Related Receptors on the Gustatory Cells of the Piglet Tongue.

Applications

Unspecified application

Species

Unspecified reactive species

Rodrigo Zamith Cunha,Ester Grilli,Andrea Piva,Cecilia Delprete,Cecilia Franciosi,Marco Caprini,Roberto Chiocchetti

Cells 13: PubMed39120329

2024

Overexpression of Toxic Poly(Glycine-Alanine) Aggregates in Primary Neuronal Cultures Induces Time-Dependent Autophagic and Synaptic Alterations but Subtle Activity Impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Steffke,Shreya Agarwal,Edor Kabashi,Alberto Catanese

Autophagy 20:2540-2555 PubMed38949671

2024

Adult-onset deactivation of autophagy leads to loss of synapse homeostasis and cognitive impairment, with implications for alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hilary Grosso Jasutkar,Elizabeth M Wasserlein,Azeez Ishola,Nicole Litt,Agnieszka Staniszewski,Ottavio Arancio,Ai Yamamoto

iScience 27:109281 PubMed38455972

2024

The decreased astrocyte-microglia interaction reflects the early characteristics of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kefu Liu,Ailikemu Aierken,Mengyao Liu,Nazakat Parhat,Wei Kong,Xingyu Yin,Gang Liu,Ding Yu,Jie Hong,Junjun Ni,Zhenzhen Quan,Xiaoyun Liu,Simei Ji,Jian Mao,Weijun Peng,Chao Chen,Yan Yan,Hong Qing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synaptophysin antibody - Synaptic Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody - Synaptic Marker (AB14692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody - Synaptic Marker (AB14692)

Western blot - Anti-Synaptophysin antibody - Synaptic Marker (AB14692)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (141)

Product promise