AB7837

Anti-Synaptophysin antibody - Synaptic Marker

Name: Anti-Synaptophysin antibody. Rabbit polyclonal (ab7837) | Abcam
Brand: Abcam Limited
SKU: AB7837
Price: 500 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(13 Publications)

Rabbit Polyclonal Synaptophysin antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 13 publications.

View Alternative Names

Synaptophysin, Major synaptic vesicle protein p38, SYP

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB7837)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB7837)

ICC/IF image of ab7837 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7837, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with a hydrophilic peptide sequence of synaptophysin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

See full target information SYP

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 23:271 PubMed40877834

2025

Anterior olfactory nucleus mediates parallel inter-bulbar pathways in rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Anan Li,Sen Jin,Yue Liu,Feilong Yu,Rafi Haddad,Fan Jia,Peng Su,Jiajia Guo,Zhijian Zhang,Qing Liu,Fuqiang Xu

Journal of neuroinflammation 21:77 PubMed38539253

2024

Liver-specific adiponectin gene therapy suppresses microglial NLRP3-inflammasome activation for treating Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Roy Chun-Laam Ng,Min Jian,Oscar Ka-Fai Ma,Ariya Weiman Xiang,Myriam Bunting,Jason Shing-Cheong Kwan,Curtis Wai-Kin Wong,Leung-Wah Yick,Sookja Kim Chung,Karen Siu-Ling Lam,Ian E Alexander,Aimin Xu,Koon-Ho Chan

NPJ Regenerative medicine 5:12 PubMed32566251

2020

Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Kourgiantaki,Dimitrios S Tzeranis,Kanelina Karali,Konstantina Georgelou,Efstathia Bampoula,Sotirios Psilodimitrakopoulos,Ioannis V Yannas,Emmanuel Stratakis,Kyriaki Sidiropoulou,Ioannis Charalampopoulos,Achille Gravanis

Nature communications 11:913 PubMed32060267

2020

MYCN amplification and ATRX mutations are incompatible in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Maged Zeineldin,Sara Federico,Xiang Chen,Yiping Fan,Beisi Xu,Elizabeth Stewart,Xin Zhou,Jongrye Jeon,Lyra Griffiths,Rosa Nguyen,Jackie Norrie,John Easton,Heather Mulder,Donald Yergeau,Yanling Liu,Jianrong Wu,Collin Van Ryn,Arlene Naranjo,Michael D Hogarty,Marcin M Kamiński,Marc Valentine,Shondra M Pruett-Miller,Alberto Pappo,Jinghui Zhang,Michael R Clay,Armita Bahrami,Peter Vogel,Seungjae Lee,Anang Shelat,Jay F Sarthy,Michael P Meers,Rani E George,Elaine R Mardis,Richard K Wilson,Steven Henikoff,James R Downing,Michael A Dyer

Journal of Alzheimer's disease : JAD 70:1025-1040 PubMed31306122

2019

Effects of Amylin Against Amyloid-β-Induced Tauopathy and Synapse Loss in Primary Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Qini Gan,Hongbo Yao,Hana Na,Heather Ballance,Qiushan Tao,Lorene Leung,Hua Tian,Haihao Zhu,Benjamin Wolozin,Wei Qiao Qiu

Biochemical pharmacology 155:264-274 PubMed30028989

2018

Novel dual incretin agonist peptide with antidiabetic and neuroprotective potential.

Applications

Unspecified application

Species

Unspecified reactive species

N M Pathak,V Pathak,V A Gault,S McClean,N Irwin,P R Flatt

Brain, behavior, and immunity 70:423-434 PubMed29604345

2018

Inflammation, insulin signaling and cognitive function in aged APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Denver,Andrew English,Paula L McClean

The Journal of endocrinology 234:255-267 PubMed28611211

2017

Metabolic and neuroprotective effects of dapagliflozin and liraglutide in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Millar,Nupur Pathak,Vadivel Parthsarathy,Anthony J Bjourson,Maurice O'Kane,Varun Pathak,R Charlotte Moffett,Peter R Flatt,Victor A Gault

Endocrinology 156:4592-603 PubMed26305888

2015

Cognitive and Disease-Modifying Effects of 11β-Hydroxysteroid Dehydrogenase Type 1 Inhibition in Male Tg2576 Mice, a Model of Alzheimer's Disease.

Applications

Species

Mouse

Karen Sooy,June Noble,Andrew McBride,Margaret Binnie,Joyce L W Yau,Jonathan R Seckl,Brian R Walker,Scott P Webster

Neuropharmacology 76 Pt A:57-67 PubMed23973293

2013

Liraglutide can reverse memory impairment, synaptic loss and reduce plaque load in aged APP/PS1 mice, a model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Paula L McClean,Christian Hölscher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Synaptophysin antibody - Synaptic Marker

Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody - Synaptic Marker (AB7837)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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