AB260417

Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker

Name: Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (ab260417) | Abcam
Brand: Abcam Limited
SKU: AB260417
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Synaptophysin antibody. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SYP aa 200 to C-terminus.

View Alternative Names

Synaptophysin, Major synaptic vesicle protein p38, SYP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (AB260417)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (AB260417)

Formalin-fixed, paraffin-embedded human pancreas tissue labeling Synaptophysin using ab260417 at 2μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (AB260417)

Formalin-fixed, paraffin-embedded human pancreatic carcinoma tissue labeling Synaptophysin using ab260417 at 2μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Protein Array - Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (AB260417)

Protein Array

Supplier Data

Protein Array - Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (AB260417)

Array of >19,000 full-length human proteins, probed with ab260417.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SYP/3551

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SYP aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08247

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

See full target information SYP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39596239

2024

p53 and the E3 Ubiquitin Ligase MDM2 in Glaucomatous Lamina Cribrosa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kealan McElhinney,Mustapha Irnaten,Jeffrey O'Callaghan,Colm O'Brien

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (AB260417)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (AB260417)

Protein Array - Anti-Synaptophysin antibody [SYP/3551] - Synaptic Marker (AB260417)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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