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Anti-Synaptophysin antibody [YE269] ab32127 is a rabbit monoclonal antibody that is used in Synaptophysin western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests- Antibody clone YE269 has been tried and trusted by researchers since 2006 and is cited in >410 publications- Specificity confirmed with SYP knockout cell line validation- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation


Images

Western blot - Anti-Synaptophysin antibody [YE269] (AB32127), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (AB32127), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (AB32127), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (AB32127), expandable thumbnail
  • Western blot - Anti-Synaptophysin antibody [YE269] (AB32127), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Tested
Expected
Tested
Mouse
Tested
Tested
Tested
Rat
Tested
Tested
Tested
Cow
Predicted
Predicted
Predicted
Donkey
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info
1/400 - 1/800
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species

Rat

Dilution info
1/400 - 1/800
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species

Human

Dilution info
1/400 - 1/800
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Donkey, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
0.1 µg/mL
Notes

-

Species

Rat

Dilution info
0.1 µg/mL
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Donkey, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/20000 - 1/100000
Notes

Can be blocked with Synaptophysin peptide (ab189853).

Species

Rat

Dilution info
1/20000 - 1/100000
Notes

Can be blocked with Synaptophysin peptide (ab189853).

Species

Human

Dilution info
1/20000 - 1/100000
Notes

Can be blocked with Synaptophysin peptide (ab189853).

Predicted
Predicted

Species

Donkey, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

2 products for Alternative Version

Target data

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Alternative names

Recommended products

Anti-Synaptophysin antibody [YE269] ab32127 is a rabbit monoclonal antibody that is used in Synaptophysin western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests- Antibody clone YE269 has been tried and trusted by researchers since 2006 and is cited in >410 publications- Specificity confirmed with SYP knockout cell line validation- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

YE269

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Browse our curated portfolio of extensively validated recombinant antibodies and assays sensitive to Tau, Beta-amyloid, and other Alzheimer’s targets of interest, with everything you need in one place.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Associated diseases and disorders

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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16 product images

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)

    Lanes 1- 2: Merged signal (red and green). Green - ab32127 observed at 38 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab32127 was shown to react with Syp in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human SYP (Synaptophysin) knockout HEK-293T cell line ab255356 (knockout cell lysate Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab263862) was used. Wild-type HEK-293T and SYP knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32127 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Synaptophysin antibody [YE269] (ab32127) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: SYP knockout HEK-293T cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)

    ab32127 staining Synaptophysin in primary mouse neurons/glia, DIV14 (prepared from E18 mouse hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab32127 at 0.1µg/ml and Anti-PSD95 antibody [K28/43] - Synaptic Marker ab192757, Mouse mono Anti-PSD95 antibody [K28/43] - Synaptic Marker. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab32127 at a dilution of 1/400.

    A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)

    ab32127 staining Synaptophysin in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab32127 at 0.1μg/ml and Anti-PSD95 antibody [K28/43] - Synaptic Marker ab192757, Mouse mono Anti-PSD95 antibody [K28/43] - Synaptic Marker. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)

    Lanes 1-3: Merged signal (red and green). Green - ab32127 observed at 38 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab32127 Anti-Synaptophysin antibody [YE269] was shown to specifically react with Synaptophysin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human SYP (Synaptophysin) knockout HEK-293T cell line ab267272 (knockout cell lysate Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab257060) was used. Wild-type and Synaptophysin knockout samples were subjected to SDS-PAGE. ab32127 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Synaptophysin antibody [YE269] (ab32127) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: SYP knockout HEK-293T cell lysate at 20 µg

    Lane 3: Human brain tissue lysate

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution

    Lane 1: Mouse brain

    Lane 2: Rat brain

    Secondary

    All lanes: HRP goat anti-rabbit at 1/1000 dilution

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling Synaptophysin with purified ab32127 at 1/100 (2.7μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)

    Lanes 1-3: Merged signal (red and green). Green - ab32127 observed at 38 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab32127 Anti-Synaptophysin antibody [YE269] was shown to specifically react with Synaptophysin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human SYP (Synaptophysin) knockout HEK-293T cell line ab267272 (knockout cell lysate Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab257060) was used. Wild-type and Synaptophysin knockout samples were subjected to SDS-PAGE. ab32127 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Synaptophysin antibody [YE269] (ab32127) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: Syp knockout HEK293T cell lysate at 20 µg

    Lane 3: Human brain tissue lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunohistochemical staining of paraffin embedded human pancreas with purified ab32127 at a dilution of 1/400.

    A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)

    All lanes: Western blot - Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution

    All lanes: Human fetal brain at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 33 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)

    All lanes: Western blot - Anti-Synaptophysin antibody [YE269] (ab32127) at 1/10000 dilution

    All lanes: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

    Predicted band size: 33 kDa

    Observed band size: 21 kDa, 22 kDa, 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunofluorescent staining of PC-12 (rat adrenal gland pheochromocytoma cell line) cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50.

    An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counterstained with DAPI.

    The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab32127 at a dilution of 1/400.

    A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Unpurified ab32127 showing positive staining in lung neuroendocrine tumor tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Unpurified ab32127 showing positive staining in medulloblastoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunohistochemical analysis of formalin fixed paraffin embedded human pancreas labelling synaptophysin with ab32127 at a concentration of 1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    ab32127 Anti-Synaptophysin antibody [YE269] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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