Rabbit Recombinant Monoclonal Synaptophysin antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.
Images
![Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--western-blot-img56086.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259)")
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img165559.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55962.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259)")
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img19391.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259)")
![Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--western-blot-img504.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (AB187259)")
Publications
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- Cell reports. Medicine 3:1006042022Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura De Vargas Roditi,Andrea Jacobs,Jan H Rueschoff,Pete Bankhead,Stéphane Chevrier,Hartland W Jackson,Thomas Hermanns,Christian D Fankhauser,Cedric Poyet,Felix Chun,Niels J Rupp,Alexandra Tschaebunin,Bernd Bodenmiller,Peter J WildPubMed 35492239
- Brain : a journal of neurology 136:1799-8152013Disease-specific molecular events in cortical multiple sclerosis lesions.
- Scientific reports 3:13452013Characterization of induced neural progenitors from skin fibroblasts by a novel combination of defined factors.Applications:ICC/IFReactive species:MouseChanghai Tian,Qiang Liu,Kangmu Ma,Yongxiang Wang,Qiang Chen,Randall Ambroz,David L Klinkebiel,Yuju Li,Yunlong Huang,Jianqing Ding,Jie Wu,Jialin C ZhengPubMed 23439431
- Brain : a journal of neurology 134:1914-242011Oxidative damage in multiple sclerosis lesions.
- Oncogene 29:335-442009Activated BRAF induces gliomas in mice when combined with Ink4a/Arf loss or Akt activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ P Robinson,M W VanBrocklin,A R Guilbeault,D L Signorelli,S Brandner,S L HolmenPubMed 19855433
- Neuroscience 165:427-352009Caprospinol reduces amyloid deposits and improves cognitive function in a rat model of Alzheimer's disease.Applications:IHCReactive species:RatL Lecanu,G Rammouz,A McCourty,E K Sidahmed,J Greeson,V PapadopoulosPubMed 19850110
- American journal of physiology. Regulatory, integrative and comparative physiology 296:R681-922009Intrauterine growth restriction due to uteroplacental insufficiency decreased white matter and altered NMDAR subunit composition in juvenile rat hippocampi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle E Schober,Robert A McKnight,Xing Yu,Christopher W Callaway,Xingrao Ke,Robert H LanePubMed 19144756
- The Journal of clinical investigation 118:3075-862008Palmitoyl protein thioesterase-1 deficiency impairs synaptic vesicle recycling at nerve terminals, contributing to neuropathology in humans and mice.Applications:WB, ICC/IFReactive species:MouseSung-Jo Kim,Zhongjian Zhang,Chinmoy Sarkar,Pei-Chih Tsai,Yi-Ching Lee,Louis Dye,Anil B MukherjeePubMed 18704195
- Investigative ophthalmology & visual science 49:3677-862008Synaptic pathology in retinoschisis knockout (Rs1-/y) mouse retina and modification by rAAV-Rs1 gene delivery.Applications:Unspecified applicationReactive species:MouseYuichiro Takada,Camasamudram Vijayasarathy,Yong Zeng,Sten Kjellstrom,Ronald A Bush,Paul A SievingPubMed 18660429
- Journal of neurochemistry 105:703-132008Delayed combinatorial treatment with flavopiridol and minocycline provides longer term protection for neuronal soma but not dendrites following global ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGrace O Iyirhiaro et. al.PubMed 18205749
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Tested | Tested |
| Rat | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Donkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Donkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Donkey | Dilution info - | Notes - |
WB
TestedTested
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Donkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Version
2 products for Alternative Product
Target data
Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Alternative names
Synaptophysin, Major synaptic vesicle protein p38, SYP
Recommended products
Rabbit Recombinant Monoclonal Synaptophysin antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.
Alternative names
Synaptophysin, Major synaptic vesicle protein p38, SYP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
YE269
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab187259 is the carrier-free version of Anti-Synaptophysin antibody [YE269] ab32127.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.
- Biological function summary
Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.
- Pathways
Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.
- Associated diseases and disorders
Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
14 product images
![Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--western-blot-img56086.jpg "Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Synaptophysin antibody [YE269] ab32127).
Lanes 1- 2: Merged signal (red and green). Green - Anti-Synaptophysin antibody [YE269] ab32127 observed at 38 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-Synaptophysin antibody [YE269] ab32127 was shown to react with Syp in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human SYP (Synaptophysin) knockout HEK-293T cell line ab255356 (knockout cell lysate Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab263862) was used. Wild-type HEK-293T and SYP knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Synaptophysin antibody [YE269] ab32127 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Synaptophysin antibody [YE269] (Anti-Synaptophysin antibody [YE269] ab32127) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: SYP knockout HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 38 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img165559.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127).
Anti-Synaptophysin antibody [YE269] ab32127 staining Synaptophysin in primary mouse neurons/glia, DIV14 (prepared from E18 mouse hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-Synaptophysin antibody [YE269] ab32127 at 0.1µg/ml and Anti-PSD95 antibody [K28/43] - Synaptic Marker ab192757, Mouse mono Anti-PSD95 antibody [K28/43] - Synaptic Marker. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55962.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified Anti-Synaptophysin antibody [YE269] ab32127 at a dilution of 1/400.
A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127).
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img19391.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127)
Anti-Synaptophysin antibody [YE269] ab32127 staining Synaptophysin in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-Synaptophysin antibody [YE269] ab32127 at 0.1?g/ml and Anti-PSD95 antibody [K28/43] - Synaptic Marker ab192757, Mouse mono Anti-PSD95 antibody [K28/43] - Synaptic Marker. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
![Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--western-blot-img504.jpg "Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Western blot - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Synaptophysin antibody [YE269] ab32127).
Lanes 1-3: Merged signal (red and green). Green - Anti-Synaptophysin antibody [YE269] ab32127 observed at 38 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-Synaptophysin antibody [YE269] ab32127 Anti-Synaptophysin antibody [YE269] was shown to specifically react with Synaptophysin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human SYP (Synaptophysin) knockout HEK-293T cell line ab267272 (knockout cell lysate Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab257060) was used. Wild-type and Synaptophysin knockout samples were subjected to SDS-PAGE. Anti-Synaptophysin antibody [YE269] ab32127 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Synaptophysin antibody [YE269] (Anti-Synaptophysin antibody [YE269] ab32127) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: SYP knockout HEK-293T cell lysate at 20 µg
Lane 3: Human brain tissue lysate
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 38 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img43602.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling Synaptophysin with purified Anti-Synaptophysin antibody [YE269] ab32127 at 1/100 (2.7μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127).![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img66323.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Clone YE269 (ab187259) has been successfully conjugated by Abcam. This image was generated using Anti-Synaptophysin antibody [YE269] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] ab196166 for protocol details.
Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] ab196166 staining Synaptophysin in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] ab196166 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product gave a positive signal in 5% formaldehyde (10 min) fixed PC12 cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137587.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Immunohistochemical staining of paraffin embedded human pancreas with purified Anti-Synaptophysin antibody [YE269] ab32127 at a dilution of 1/400.
A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127).
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img68261.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Clone YE269 (ab187259) has been successfully conjugated by Abcam. This image was generated using Anti-Synaptophysin antibody [YE269] (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] ab196379 for protocol details.
Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] ab196379 staining Synaptophysin in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] ab196379 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img133252.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
This image is courtesy of an anonymous customer review.Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Anti-Synaptophysin antibody [YE269] ab32127 staining synaptophysin in human iPS cell derived neurons by immunocytochemistry/immmunofluorescence.
Samples were fixed with paraformaldehyde and blocked with 1% serum for 30 minutes at room temperature. Samples were incubated with primary antibody at 1/250 dilution for 1 hour. Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150061 was used as the secondary antibody at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127).
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img108335.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Immunofluorescent staining of PC-12 (rat adrenal gland pheochromocytoma cell line) cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified Anti-Synaptophysin antibody [YE269] ab32127 at a dilution of 1/50.
An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counterstained with DAPI.
The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48912.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified Anti-Synaptophysin antibody [YE269] ab32127 at a dilution of 1/400.
A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22172.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
Unpurified Anti-Synaptophysin antibody [YE269] ab32127 showing positive staining in lung neuroendocrine tumor tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Synaptophysin antibody [YE269] ab32127).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107204.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
This IHC data was generated using the same anti-Synaptophysin antibody clone, YE269, in a different buffer formulation (cat# Anti-Synaptophysin antibody [YE269] ab32127).
Unpurified Anti-Synaptophysin antibody [YE269] ab32127 showing positive staining in Medulloblastoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img43602.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img66323.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137587.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img68261.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img133252.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
![Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunocytochemistry-immunofluorescence-img108335.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48912.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22172.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259), expandable thumbnail](https://content.abcam.com/products/images/synaptophysin-antibody-ye269-bsa-and-azide-free-ab187259--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107204.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)")
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