Anti-Synaptopodin antibody [EPR23922-79] 20 ul size (ab259976)

Rabbit Recombinant Monoclonal Synaptopodin antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

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Images

Western blot - Anti-Synaptopodin antibody [EPR23922-79] (AB259976), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	WB	IHC-Fr	IHC-P
Human	Expected	Not recommended	Not recommended	Expected	Tested
Mouse	Tested	Not recommended	Tested	Tested	Tested
Rat	Tested	Not recommended	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Species Rat	Dilution info 1/100	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information SYNPO

Function

Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity (By similarity).

Alternative names

KIAA1029, SYNPO, Synaptopodin

Recommended products

Rabbit Recombinant Monoclonal Synaptopodin antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23922-79
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Synaptopodin also known as SYNPO is an actin-associated protein with a molecular mass of approximately 100 kDa. It plays a mechanical role by associating and bundling actin filaments important for cytoskeletal dynamics. Synaptopodin gets expressed mainly in podocytes of the kidney as well as in certain neurons particularly found in dendritic spines and telencephalic dendrites. This protein is sensitive to calcium signaling which influences its interaction with the actin cytoskeleton.

Biological function summary

The actin-modulating effects of Synaptopodin contribute to the regulation of cell shape and motility. Synaptopodin is essential for the formation of the dendritic spine apparatus working as a part of a protein complex that stabilizes dendritic spines which are key to synaptic plasticity and signal transduction. The protein can also influence the podocyte function in the kidney maintaining the structure of foot processes that are fundamental for the kidney filtration barrier.

Pathways

Synaptopodin plays an important role in the maintenance of synaptic plasticity and the actin cytoskeleton pathway which affects both neuronal plasticity and kidney function. Within these pathways Synaptopodin interacts with proteins like alpha-actinin and myosin-Va forming linkages that stabilize actin filaments and regulate cytoskeletal organization. The protein contributes to the protection of neurons and kidney glomeruli through signaling pathways that affect actin dynamic balance.

Associated diseases and disorders

Synaptopodin has significant implications in nephrotic syndrome and Alzheimer's disease. In nephrotic syndrome alterations in Synaptopodin expression or function can lead to podocyte injury contributing to kidney dysfunction. In Alzheimer's disease Synaptopodin modulation of spine morphology may impact synaptic connectivity and cognitive function. The protein interacts with nephrin in kidney pathology and potentially affects tau protein signaling in Alzheimer's illustrating its vital role in both neurological and renal health.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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15 product images

Western blot - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight is consistent to what has been described in literatures (PMID:18724379, PMID:15841212)
Negative control: liver (PMID:11595771)
Exposure time: 15 seconds
All lanes: Western blot - Anti-Synaptopodin antibody [EPR23922-79] (ab259976) at 1/1000 dilution
Lane 1: Mouse hippocampus tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 40 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 99 kDa
Observed band size: 100 kDa
Western blot - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight is consistent to what has been described in literatures (PMID:18724379, PMID:15841212)
Negative control: liver (PMID:11595771)
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 92 seconds
All lanes: Western blot - Anti-Synaptopodin antibody [EPR23922-79] (ab259976) at 1/1000 dilution
Lane 1: Rat hippocampus tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 40 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 99 kDa
Observed band size: 100 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling Synaptopodin with ab259976 at 1/500 (0.936 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining mainly in glomeruli of mouse kidney (PMID: 22475818, PMID: 15841212). The section was incubated with ab259976 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Synaptopodin with ab259976 at 1/500 (0.936 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse cerebrum (PMID: 15841212). The section was incubated with ab259976 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling Synaptopodin with ab259976 at 1/500 (0.936 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining mainly in glomeruli of rat kidney. The section was incubated with ab259976 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Synaptopodin with ab259976 at 1/500 (0.936 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in rat cerebrum. The section was incubated with ab259976 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Synaptopodin with ab259976 at 1/500 (0.936 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining mainly in glomeruli of human kidney. The section was incubated with ab259976 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling Synaptopodin with ab259976 at 1/500 (0.936 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human cerebrum. The section was incubated with ab259976 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling Synaptopodin with ab259976 at 1/100 (4.68 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebral cortex is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling Synaptopodin with ab259976 at 1/100 (4.68 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse hippocampus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Synaptopodin was immunoprecipitated from 0.35 mg rat hippocampus tissue lysate 10μg with ab259976 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259976 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat hippocampus tissue lysate 10μg
Lane 2: ab259976 IP in rat hippocampus tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259976 in rat hippocampus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
All lanes: Immunoprecipitation - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Predicted band size: 99 kDa
Observed band size: 100 kDa
Immunohistochemistry (Frozen sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Synaptopodin with ab259976 at 1/100 (4.68 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on glomerulus of mouse kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling Synaptopodin with ab259976 at 1/100 (4.68 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebral cortex tissue labeling Synaptopodin with ab259976 at 1/100 (4.68 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebral cortex is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Synaptopodin antibody [EPR23922-79] (ab259976)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Synaptopodin with ab259976 at 1/100 (4.68 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on glomerulus of rat kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).