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AB131548

Anti-Synaptotagmin 1 + 2 antibody

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Rabbit Polyclonal SYT1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human SYT1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

SVP65, SYT, SYT1, Synaptotagmin-1, Synaptotagmin I, p65, SytI

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin 1 + 2 antibody (AB131548)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin 1 + 2 antibody (AB131548)

Immunofluorescent analysis of methanol-fixed HeLa cells labelling Synaptotagmin 1 + 2 with ab131548 at 1/100 dilution.

Western blot - Anti-Synaptotagmin 1 + 2 antibody (AB131548)
  • WB

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Western blot - Anti-Synaptotagmin 1 + 2 antibody (AB131548)

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Western blot - Anti-Synaptotagmin 1 + 2 antibody (ab131548) at 1/500 dilution

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HeLa cell extract

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human SYT2 conjugated to Keyhole Limpet Haemocyanin. Database link Q8N9I0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptotagmin 1 and Synaptotagmin 2 also known as SYT1 and SYT2 respectively play essential roles in the mechanics of synaptic transmission. These proteins function as calcium sensors that trigger neurotransmitter release primarily by promoting vesicle fusion with presynaptic membranes. SYT1 has a mass of 47 kDa while SYT2 weighs around 49 kDa. Both proteins localize in neuronal tissues especially at the synaptic junctions indicating their role in nerve signaling.
Biological function summary

Synaptotagmin proteins facilitate exocytosis by interacting with the SNARE complex a critical component in synapse function. These proteins bind calcium ions which lead to conformational changes and the subsequent fusion of synaptic vesicles with the plasma membrane. Part of the extended family of synaptotagmins SYT1 and SYT2 participate in important neural processes leveraging their ability to coordinate responses between electrical inputs and chemical outputs within neurons.

Pathways

The molecular function of Synaptotagmin 1 and 2 centers around their role in neurotransmitter release pathways. These proteins are integral to the calcium-dependent exocytosis pathway and the vesicle-mediated transport pathway. They directly interact with proteins like syntaxin and SNAP-25 within the SNARE complex. Their activity ensures a well-coordinated release of neurotransmitters influencing synaptic plasticity and overall neural network communication.

Synaptotagmin 1 and 2 have connections to neurological conditions such as epilepsy and schizophrenia. Their malfunction or altered expression can disrupt normal synaptic signaling and may lead to an imbalance in neurotransmitter release. In epilepsy for example they are potentially linked with hyperexcitability due to ineffective synapse regulation. Additionally changes in their interaction with SNARE-related proteins might affect neural communication contributing to the pathophysiology of schizophrenia.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed : 23999003).
See full target information SYT1

Additional targets

SYT2
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