AB133694

Anti-Synaptotagmin antibody

Name: Anti-Synaptotagmin antibody (ab133694) | Abcam
Brand: Abcam Limited
SKU: AB133694

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(1 Publication)

Rabbit Polyclonal SYT2 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SYT2.

View Alternative Names

Synaptotagmin-2, Synaptotagmin II, SytII, SYT2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin antibody (AB133694)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin antibody (AB133694)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex neurons labelling Synaptotagmin with ab133694 at 15 μg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-Synaptotagmin antibody (AB133694)

All lanes:

Western blot - Anti-Synaptotagmin antibody (ab133694) at 1/500 dilution

Lane 1:

Extract from 293 cells treated with Forskolin (40 nM 30')

Lane 2:

Extract from 293 cells treated with Forskolin (40 nM 30') with immunizing peptide

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SYT2. The exact immunogen used to generate this antibody is proprietary information.

Q8N9I0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "15 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store undiluted

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptotagmin acts as a critical calcium sensor in the process of neurotransmitter release within synaptic vesicles. Also known as Syt synaptotagmin exhibits a mass of approximately 60 kDa. It is primarily expressed in neuronal tissues as well as in vesicles associated with secretory pathways. Synaptotagmin functionality relies on its ability to bind calcium ions facilitating the rapid fusion of vesicles with the plasma membrane during neurotransmission.

Biological function summary

Synaptotagmins play a central role in synaptic transmission and vesicular trafficking. They belong to a family of proteins involved in exocytosis where they interact as part of the SNARE complex. Synaptotagmin acts as a pivotal component by triggering vesicle fusion when it detects an influx of calcium ions. This process ultimately leads to the release of neurotransmitters into the synaptic cleft impacting numerous neural communication mechanisms.

Pathways

Synaptotagmins contribute significantly to synaptic vesicle cycling and calcium-mediated neuronal transmission. This protein is part of the synaptic vesicle cycle influencing pathways that regulate neurotransmitter release in response to calcium influx. It associates with proteins such as SNAP-25 and syntaxin working together to support vesicle docking and fusion processes essential for efficient synaptic signal transmission.

Synaptotagmins have connections to conditions like schizophrenia and bipolar disorder due to their role in neurotransmitter release regulation. Aberrant function or expression of synaptotagmins may influence synaptic activity contributing to these neuropsychiatric disorders. In some cases synaptotagmin dysfunction alters interactions with other proteins like complexins which impacts synaptic vesicle exocytosis and may relate to neurological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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Target data

Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).

See full target information SYT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:110 PubMed38598023

2024

Hypoxic microenvironment-induced exosomes confer temozolomide resistance in glioma through transfer of pyruvate kinase M2.

Applications

Unspecified application

Species

Unspecified reactive species

Guofu Li,Ziyu Xiong,Ying Li,Cong Yan,Yingying Cheng,Yuwen Wang,Jingwei Li,Zifeng Dai,Dongdong Zhang,Wenzhong Du,Chunyang Men,Changbin Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synaptotagmin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin antibody (AB133694)

Western blot - Anti-Synaptotagmin antibody (AB133694)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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