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Mouse Monoclonal SYT2 antibody. Suitable for WB, ICC/IF, IP and reacts with Rat, Mouse samples. Cited in 23 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Syt1.


Images

Western blot - Anti-Synaptotagmin antibody [ASV30] (AB13259), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin antibody [ASV30] (AB13259), expandable thumbnail
  • Immunoprecipitation - Anti-Synaptotagmin antibody [ASV30] (AB13259), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Full Length Protein corresponding to Rat Syt1. The exact immunogen used to generate this antibody is proprietary information. Database link P21707

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIP
Mouse
Tested
Expected
Tested
Rat
Tested
Tested
Expected

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Rat
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties (PubMed:7961887). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (PubMed:7961887). Plays a role in dendrite formation by melanocytes (By similarity). (Microbial infection) Receptor for C.botulinum neurotoxin type B (BoNT/B, botB); interaction is improved in the presence of gangliosides (PubMed:14504267). The toxin binds via the vesicular domain (residues 47-60) (PubMed:14504267, PubMed:17167418, PubMed:23807078). (Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG); gangliosides are not required for (or only very slightly improve) binding to a membrane-anchored receptor fragment (PubMed:20219474). The toxin binds via the vesicular domain (residues 47-55) (PubMed:20219474).

Alternative names

Synaptotagmin-2, Inositol polyphosphate-binding protein, Synaptotagmin II, IP4-binding protein, IP4BP, SytII, Syt2

Recommended products

Mouse Monoclonal SYT2 antibody. Suitable for WB, ICC/IF, IP and reacts with Rat, Mouse samples. Cited in 23 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Syt1.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Full Length Protein corresponding to Rat Syt1. The exact immunogen used to generate this antibody is proprietary information. Database link P21707
Clone number
ASV30
Purification technique
Affinity purification Protein G
Specificity

ab13259 is not specific to SYT2 but is a pan-synaptotagmin antibody. It does not distinguish between the Synaptotagmin family of proteins.

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 5th July 2019. Lot numbers higher than GR3258922 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Synaptotagmin acts as a critical calcium sensor in the process of neurotransmitter release within synaptic vesicles. Also known as Syt synaptotagmin exhibits a mass of approximately 60 kDa. It is primarily expressed in neuronal tissues as well as in vesicles associated with secretory pathways. Synaptotagmin functionality relies on its ability to bind calcium ions facilitating the rapid fusion of vesicles with the plasma membrane during neurotransmission.

Biological function summary

Synaptotagmins play a central role in synaptic transmission and vesicular trafficking. They belong to a family of proteins involved in exocytosis where they interact as part of the SNARE complex. Synaptotagmin acts as a pivotal component by triggering vesicle fusion when it detects an influx of calcium ions. This process ultimately leads to the release of neurotransmitters into the synaptic cleft impacting numerous neural communication mechanisms.

Pathways

Synaptotagmins contribute significantly to synaptic vesicle cycling and calcium-mediated neuronal transmission. This protein is part of the synaptic vesicle cycle influencing pathways that regulate neurotransmitter release in response to calcium influx. It associates with proteins such as SNAP-25 and syntaxin working together to support vesicle docking and fusion processes essential for efficient synaptic signal transmission.

Associated diseases and disorders

Synaptotagmins have connections to conditions like schizophrenia and bipolar disorder due to their role in neurotransmitter release regulation. Aberrant function or expression of synaptotagmins may influence synaptic activity contributing to these neuropsychiatric disorders. In some cases synaptotagmin dysfunction alters interactions with other proteins like complexins which impacts synaptic vesicle exocytosis and may relate to neurological conditions.

Product promise

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3 product images

  • Western blot - Anti-Synaptotagmin antibody [ASV30] (ab13259), expandable thumbnail

    Western blot - Anti-Synaptotagmin antibody [ASV30] (ab13259)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Synaptotagmin antibody [ASV30] (ab13259) at 1/1000 dilution

    Lane 2: Lysates prepared from mouse brain

    Lane 3: Lysates prepared from rat brain

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin antibody [ASV30] (ab13259), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin antibody [ASV30] (ab13259)

    ICC/IF image of ab13259 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13259, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

    This image was generated using the ascites version of the product.

  • Immunoprecipitation - Anti-Synaptotagmin antibody [ASV30] (ab13259), expandable thumbnail

    Immunoprecipitation - Anti-Synaptotagmin antibody [ASV30] (ab13259)

    Synaptotagmin was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5μg of Mouse monoclonal to Synaptotagmin and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13259.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
    Band: 64kDa; Synaptotagmin

    This image was generated using the ascites version of the product.

    All lanes: Immunoprecipitation - Anti-Synaptotagmin antibody [ASV30] (ab13259)

    Predicted band size: 47 kDa

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Product protocols

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