Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Synaptotagmin VII with ab311843 at 1/2000 (0.265 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cererbrum. The section was incubated with ab311843 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Synaptotagmin VII with ab311843 at 1/50 (10.6 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse liver (PMID : 7791877). The nuclear counterstain was DAPI (Blue). The section was incubated with ab311843 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Synaptotagmin VII with ab311843 at 1/50 (10.6 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on rat liver (PMID : 7791877). The nuclear counterstain was DAPI (Blue). The section was incubated with ab311843 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Synaptotagmin VII with ab311843 at 1/50 (10.6 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab311843 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Synaptotagmin VII with ab311843 at 1/2000 (0.265 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cererbrum.The section was incubated with ab311843 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Synaptotagmin VII with ab311843 at 1/2000 (0.265 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cererbrum (PMID : 29593071). The section was incubated with ab311843 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Synaptotagmin VII with ab311843 at 1/50 (10.6 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab311843 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Synaptotagmin VII with ab311843 at 1/2000 (0.265 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on mouse liver. The section was incubated with ab311843 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• WB

Supplier Data

Western blot - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver, testis(PMID : 7791877). Samples are non-boiled as boiling may cause protein aggregation. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 7791877). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-Synaptotagmin VII antibody [EPR27957-6] (<a href='/en-us/products/primary-antibodies/synaptotagmin-vii-antibody-epr27957-6-ab311843'>ab311843</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat cerebellum tissue lysate at 20 µg

Lane 3:

Rat olfactory bb tissue lysate 20 at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Lane 5:

Rat testis tissue lysate at 20 µg

Lane 6:

Human cerebellum tissue lysate at 20 µg

Lane 7:

human hypothalamus tissue lysate at 20 µg

Lane 8:

human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver, testis(PMID : 7791877). Samples are non-boiled as boiling may cause protein aggregation. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 7791877). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-Synaptotagmin VII antibody [EPR27957-6] (<a href='/en-us/products/primary-antibodies/synaptotagmin-vii-antibody-epr27957-6-ab311843'>ab311843</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse hippocampus tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-Synaptotagmin VII antibody [EPR27957-6] - BSA and Azide free (AB311844)

This data was developed using ab311843, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody has weak cross reactivity with mouse SYT1 and SYT2. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-Synaptotagmin VII antibody [EPR27957-6] (<a href='/en-us/products/primary-antibodies/synaptotagmin-vii-antibody-epr27957-6-ab311843'>ab311843</a>) at 1/1000 dilution

Lane 1:

His-tagged mouse SYT7 recombinant protein at 10 ng

Lane 2:

His-tagged mouse SYT2 recombinant protein at 10 ng

Lane 3:

His-tagged mouse SYT1 recombinant protein at 10 ng

Lane 4:

His-tagged mouse SYT3 recombinant protein at 10 ng

Lane 5:

His-tagged mouse SYT4 recombinant protein at 100 ng

Lane 6:

His-tagged mouse SYT5 recombinant protein at 100 ng

Lane 7:

His-tagged mouse SYT6 recombinant protein at 10 ng

Lane 8:

His-tagged mouse SYT9 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 37s