AB60199

Anti-Syndecan-1 antibody

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(9 Publications)

Rabbit Polyclonal Syndecan-1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 9 publications.

View Alternative Names

CD138, SDC, SDC1, Syndecan-1, SYND1

2 Images

Unknown

Western blot - Anti-Syndecan-1 antibody (AB60199)

The observed band size is larger than predicted due to haparan sulfate and chondroitin sulfate modifications.

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Western blot - Anti-Syndecan-1 antibody (ab60199) at 4 µg/mL

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Mouse tissue lysate

Predicted band size: 32 kDa

Observed band size: 100 kDa

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PubMed

Western blot - Anti-Syndecan-1 antibody (AB60199)

Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.

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Western blot - Anti-Syndecan-1 antibody (ab60199) at 1/2000 dilution

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lysate prepared from ScN2a cells.

Secondary

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peroxidase-conjugated secondary

Predicted band size: 32 kDa

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Image from Taylor DR et al, PLoS Pathog. 2009 Nov;5(11):e1000666. Epub 2009 Nov 20, Fig 6.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human SDC1. The exact immunogen used to generate this antibody is proprietary information.

P18827

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3643R Syndecan-1 Antibody. 3643R-100 is the same size as the 100 µg size of ab60199.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.

Biological function summary

Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.

Pathways

Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.

Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the SDC1 gene is a cell surface proteoglycan with heparan sulfate and chondroitin sulfate, linking the cytoskeleton to the interstitial matrix. It regulates exosome biogenesis alongside SDCBP and PDCD6IP. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SDC1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35008785

2021

Inhibition of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade and Promotes Recovery after Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Guang Yu,Vimala Bondada,Hina Iqbal,Kate L Moore,John C Gensel,Subbarao Bondada,James W Geddes

Cancer management and research 13:2885-2896 PubMed33833571

2021

circCEP128 Knockdown Suppresses Bladder Cancer Progression via Regulating microRNA-515-5p/SDC1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghui Cao,Chan Zhang,Xiangyong Tian,Gaopeng Jing,Xiaolin Zhou,Tianzhong Yan

Cell biology international 44:894-904 PubMed31868265

2020

Phosphorylation of Dab2 is involved in inhibited VEGF-VEGFR-2 signaling induced by downregulation of syndecan-1 in glomerular endothelial cell.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Jing,Wu Jia-Jun,Yuan Wei-Jie

The Journal of clinical investigation 125:2877-90 PubMed26098211

2015

Interferon-induced mechanosensing defects impede apoptotic cell clearance in lupus.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Li,Yang-Xin Fu,Qi Wu,Yong Zhou,David K Crossman,PingAr Yang,Jun Li,Bao Luo,Laurence M Morel,Janusz H Kabarowski,Hideo Yagita,Carl F Ware,Hui-Chen Hsu,John D Mountz

PloS one 9:e89824 PubMed24587062

2014

Syndecan-1 regulates vascular smooth muscle cell phenotype.

Applications

Unspecified application

Species

Mouse

Somali Chaterji,Christoffer H Lam,Derek S Ho,Daniel C Proske,Aaron B Baker

Molecular human reproduction 20:567-78 PubMed24478463

2014

Syndecan-1 knockdown in endometrial epithelial cells alters their apoptotic protein profile and enhances the inducibility of apoptosis.

Applications

Unspecified application

Species

Human

S J Boeddeker,D M Baston-Buest,O Altergot-Ahmad,J S Kruessel,A P Hess

Investigative ophthalmology & visual science 54:6373-81 PubMed23989188

2013

An investigative peptide-acyclovir combination to control herpes simplex virus type 1 ocular infection.

Applications

Unspecified application

Species

Unspecified reactive species

Paul J Park,Thessicar E Antoine,Asim V Farooq,Tibor Valyi-Nagy,Deepak Shukla

Development (Cambridge, England) 138:4523-33 PubMed21903674

2011

Alpha sarcoglycan is required for FGF-dependent myogenic progenitor cell proliferation in vitro and in vivo.

Applications

Species

Mouse

Marco Cassano,Arianna Dellavalle,Francesco Saverio Tedesco,Mattia Quattrocelli,Stefania Crippa,Flavio Ronzoni,Agnese Salvade,Emanuele Berardi,Yvan Torrente,Giulio Cossu,Maurilio Sampaolesi

PLoS pathogens 5:e1000666 PubMed19936054

2009

Glypican-1 mediates both prion protein lipid raft association and disease isoform formation.

Applications

Species

Mouse

David R Taylor,Isobel J Whitehouse,Nigel M Hooper

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Syndecan-1 antibody

Western blot - Anti-Syndecan-1 antibody (AB60199)

Western blot - Anti-Syndecan-1 antibody (AB60199)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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