Anti-Syndecan-1 antibody [EPR6454] ab128936 is a rabbit monoclonal antibody that is used in Syndecan-1 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR6454 is cited in over 60 publications
- One antibody for all your Syndecan-1 staining, use in Syndecan-1 western blotting, IHC, immunofluorescence and flow cytometry
New 20 ul size available
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected | Expected |
Rat | Expected | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/8000 | Notes For unpurified, use 1/500 - 1/1000. Based on negative staining of mouse and rat spleen and stomach tissues in IHC-P we believe this antibody is unsuitable for IHC-P with mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (By similarity). Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413). Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (By similarity).
SDC, SDC1, Syndecan-1, SYND1
Anti-Syndecan-1 antibody [EPR6454] ab128936 is a rabbit monoclonal antibody that is used in Syndecan-1 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR6454 is cited in over 60 publications
- One antibody for all your Syndecan-1 staining, use in Syndecan-1 western blotting, IHC, immunofluorescence and flow cytometry
New 20 ul size available
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR6454
Affinity purification Protein A
Based on negative staining of mouse and rat spleen and stomach tissues in IHC-P we believe this antibody is unsuitable for IHC-P with mouse and rat. We believe the antibody is suitable for Western blot with mouse and rat based on data from brain, heart, kidney, and spleen lysates. Please contact our Scientific Support team for more information.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Stable for 12 months at -20°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.
Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.
Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.
Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical staining of paraffin embedded human colon with purified ab128936 at a working dilution of 1/8000. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry analysis of A431 cell line labeling Syndecan-1 with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 2.5 μg/ml (top left). Cells were fixed with 4% paraformaldehyde, permaeabilized with 0.1% tritonX-100. DAPI nuclear stain. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 2 μg/ml. Confocal image showing membranous staining on A431 cell line.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/5000 dilution
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: PC-12 whole cell lysate at 20 µg
Lane 3: Mouse heart tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 100 kDa, 35 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/10000 dilution
Lane 1: Ramos whole cell lysate at 10 µg
Lane 2: Raji whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 100 kDa, 35 kDa
All lanes: Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution
Lane 1: Ramos cell lysate at 10 µg
Lane 2: Raji cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: BxPC-3 cell lysate at 10 µg
Lane 5: Fetal kidney lysate at 10 µg
Lane 6: PC12 cell lysate at 10 µg
All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa, 65-100 kDa
Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Unpurified ab128936 showing positive staining in Normal human skin tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Unpurified ab128936 showing positive staining in normal human tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Unpurified ab128936 showing positive staining in human Plasmacytoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Unpurified ab128936 showing negative staining in human Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) labelling -Syndecan-1 with ab128936 at 1/50 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunocytochemistry analysis of paraformaldehyde-fixed 0.05% Tween 20 permeabilized human Kidney H9 Organoid cells staining with ab128936 at 1/500 dilution. Secondary antibody was Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed at 1/300 dilution. Samples were incubated with the primary antibody with Sea Block for 14 hours at 4°C. Blocking was done using 100% Sea Block for 30 minutes at 22°C.
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