Rabbit Recombinant Monoclonal Syndecan 3 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Syndecan 3 antibody [EPR10648] - BSA and Azide free (AB249207), expandable thumbnail](https://content.abcam.com/products/images/syndecan-3-antibody-epr10648-bsa-and-azide-free-ab249207--western-blot-img116018.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Syndecan 3 antibody [EPR10648] - BSA and Azide free (AB249207)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Predicted | Not recommended | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
Alternative names
KIAA0468, SDC3, Syndecan-3, SYND3
Recommended products
Rabbit Recombinant Monoclonal Syndecan 3 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10648
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249207 is the carrier-free version of Anti-Syndecan 3 antibody [EPR10648] ab155952.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syndecan 3 also known as N-syndecan is a heparan sulfate proteoglycan with a molecular mass of around 120-150 kDa. It has a core protein containing three heparan sulfate chains essential for its function. Expressed mainly in the nervous system Syndecan 3 shows significant concentration in neurons and glial cells. This proteoglycan plays an important role in cell-cell and cell-matrix interactions and contributes to neural developmental processes.
- Biological function summary
Syndecan 3 modulates cellular behaviors including proliferation differentiation and migration. It functions as a co-receptor for various growth factors and adhesion molecules. This interaction facilitates signaling pathways that influence neural structure and function. Syndecan 3 forms complexes with other cell surface molecules which enhance its ability to mediate cellular responses. These interactions are essential for the development and maintenance of the nervous system.
- Pathways
Syndecan 3 interacts within important signaling cascades such as the Wnt and fibroblast growth factor (FGF) pathways. The Wnt pathway involves the regulation of gene expression critical for cellular processes while FGF pathways direct cell division and differentiation. Syndecan 3's interaction with proteins like FGF receptors highlights its role in these pathways. It aids in modulating the activity of related proteins coordinating integrative signals that ensure proper neuronal development.
- Associated diseases and disorders
Syndecan 3 relates to neurodegenerative conditions like Alzheimer's Disease and developmental disorders such as autism. In Alzheimer's Disease its association with amyloid beta precursor protein (APP) suggests a role in amyloid plaque formation. In autism changes in the expression of Syndecan 3 may influence neural connectivity and communication. These interactions emphasize the importance of Syndecan 3 in maintaining neuronal integrity and highlight its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Syndecan 3 antibody [EPR10648] - BSA and Azide free (ab249207), expandable thumbnail](https://content.abcam.com/products/images/syndecan-3-antibody-epr10648-bsa-and-azide-free-ab249207--western-blot-img116018.jpg "Western blot - Anti-Syndecan 3 antibody [EPR10648] - BSA and Azide free (ab249207)")
Western blot - Anti-Syndecan 3 antibody [EPR10648] - BSA and Azide free (ab249207)
This data was developed using Anti-Syndecan 3 antibody [EPR10648] ab155952, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Syndecan 3 antibody [EPR10648] (Anti-Syndecan 3 antibody [EPR10648] ab155952) at 1/1000 dilution
Lane 1: SH-SY5Y cell lysate at 10 µg
Lane 2: Human spleen tissue lysate at 10 µg
Lane 3: Human adrenal gland tissue lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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