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AB286154

Anti-Syndecan 4 antibody

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(1 Publication)

Rabbit Polyclonal Syndecan 4 antibody. Suitable for IHC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SDC4.

View Alternative Names

Syndecan-4, SYND4, Amphiglycan, Ryudocan core protein, SDC4

2 Images
Immunohistochemistry - Anti-Syndecan 4 antibody (AB286154)
  • IHC

Supplier Data

Immunohistochemistry - Anti-Syndecan 4 antibody (AB286154)

Immunohistochemistry of formalin fixed, paraffin-embedded human colon tissue after heat-induced antigen retrieval. 5 μg/ml of antibody was used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Western blot - Anti-Syndecan 4 antibody (AB286154)
  • WB

Supplier Data

Western blot - Anti-Syndecan 4 antibody (AB286154)

100 μg Jurkat cell lysate was loaded. Blocking/Dilution buffer : 5% NFDM (1 hr at RT) / TBST. ab286154 was used at 1/1000 dilution), O/N at 4 °C. Goat Anti-Rabbit IgG peroxidase conjugated used at 1/10,000 dilution, 1 hr at RT.

All lanes:

Western blot - Anti-Syndecan 4 antibody (ab286154) at 1/1000 dilution

All lanes:

Jurkat cell lysate at 100 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG peroxidase conjugated at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC, WB

applications

Immunogen

Synthetic Peptide within Human SDC4. The exact immunogen used to generate this antibody is proprietary information.

P31431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHC-species-checked": "predicted", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHC-species-checked": "predicted", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3644 Syndecan-4 Antibody. 3644-100 is the same size as the 100 µg size of ab286154.
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syndecan 4 also known as SDC4 is a transmembrane heparan sulfate proteoglycan with a molecular mass of around 30 kDa. It functions mechanically by interacting with the extracellular matrix via its heparan sulfate chains which are critical for binding growth factors and other matrix molecules. Syndecan 4 is ubiquitously expressed in many cell types including fibroblasts and epithelial cells playing a role in cell adhesion and migration.
Biological function summary

Syndecan 4 contributes to cell signaling and cytoskeletal organization. This proteoglycan acts as an essential co-receptor for various growth factors facilitating their interaction with their receptors on the cell surface. By doing so it supports cell proliferation and repair processes and it's also known to interact with integrins to modulate cell adhesion. Syndecan 4 does not function alone; it forms part of complexes with other cell surface receptors and interacts with key signaling molecules inside cells.

Pathways

Syndecan 4 is integral in the regulation of several signal transduction pathways such as the protein kinase C (PKC) and Rho family GTPase pathways. These pathways are essential for modulating cellular responses to mechanical stress and changes in the extracellular environment. Syndecan 4 works closely with proteins such as fibronectin and integrins within these pathways to distribute mechanical signals appropriately across the cell membrane linking the extracellular matrix to intracellular processes.

Syndecan 4 has strong associations with conditions like cancer and arthritis. In cancer altered Syndecan 4 expression can impact tumor cell proliferation and metastasis often in concert with matrix metalloproteinases which remodel the extracellular matrix environment to facilitate invasion. In arthritis Syndecan 4 plays a role in inflammatory processes where its interactions with cytokines and other cell-surface proteins can influence cartilage degradation and joint inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface proteoglycan which regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed : 22660413).
See full target information SDC4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e18600 PubMed37576224

2023

Syndecan-4 is the key proteoglycan involved in mediating sepsis-associated lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Zhu,Xiaoyan Ling,Hongmei Zhou,Junran Xie
View all publications
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Product promise

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