AB247491

Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SynGAP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

KIAA1938, SYNGAP1, Ras/Rap GTPase-activating protein SynGAP, Neuronal RasGAP, Synaptic Ras GTPase-activating protein 1, Synaptic Ras-GAP 1

2 Images

Unknown

Western blot - Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (AB247491)

This data was developed using ab77235, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SynGAP antibody [EPR2883Y] (<a href='/en-us/products/primary-antibodies/syngap-antibody-epr2883y-ab77235'>ab77235</a>) at 1/1000 dilution

All lanes:

fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 148 kDa

Observed band size: 147 kDa,50 kDa,55 kDa

false

Lab

Western blot - Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (AB247491)

This data was developed using ab77235, the same antibody clone in a different buffer formulation.

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab77235 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

The doublet observed in the Western blot image is consistent with the literature (PMID : 9581761).

All lanes:

Western blot - Anti-SynGAP antibody [EPR2883Y] (<a href='/en-us/products/primary-antibodies/syngap-antibody-epr2883y-ab77235'>ab77235</a>) at 1/1000 dilution

Lane 1:

Hippocampus (Rat) Tissue Lysate at 20 µg

Lane 2:

Cortex (Rat) Tissue Lysate at 20 µg

Lane 3:

Hippocampus (Mouse) Tissue Lysate at 20 µg

Lane 4:

Cortex (Mouse) Tissue Lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 148 kDa

Observed band size: 130 kDa,148 kDa

true

Exposure time: 8min

Unconjugated

Anti-SynGAP antibody [EPR2883Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2883Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247491 is the carrier-free version of ab77235.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SynGAP also known as Synaptic Ras GTPase-activating protein 1 has a molecular weight of about 138 kDa. It predominantly expresses in the brain especially in the post-synaptic density of neurons. SynGAP acts mechanically by regulating Ras and Rap GTPase signaling through its GTPase-activating protein (GAP) domain. This protein modulates synaptic strength and plasticity by influencing the activity of these GTPases which are pivotal for intracellular signaling.

Biological function summary

SynGAP influences synaptic function and plasticity. It forms part of the NMDA receptor complex linking it to various synaptic signaling pathways. SynGAP modulates excitatory synaptic transmission by controlling the levels of Ras and Rap GTPases which impact the phosphorylation of AMPA receptors. Through these activities it maintains proper synaptic strength and structural integrity essential for learning and memory processes.

Pathways

SynGAP interfaces with signaling networks critical for neuronal function such as the MAPK/ERK and PI3K/Akt pathways. In these pathways SynGAP regulates Ras-related protein kinase activity influencing downstream effects on gene expression and cellular survival. The protein's interaction with other partners like the PSD-95 and other MAGUK family proteins highlights its central role in controlling synaptic signaling cascades.

Researchers link SynGAP to neurodevelopmental disorders like intellectual disability and autism spectrum disorders. Mutations or disruptions in SynGAP levels correlate with altered synaptic function and contribute to these conditions. SynGAP also interacts with proteins such as SHANK3 which connects to synaptic abnormalities observed in these disorders. Understanding its role in such conditions can provide insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. Exhibits dual GTPase-activating specificity for Ras and Rap. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits (By similarity).

See full target information SYNGAP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free

Western blot - Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (AB247491)

Western blot - Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (AB247491)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Secondary Antibodies

Primary Antibodies

Product promise