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AB317190

Anti-SYNGAP1 antibody [HL2292]

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Rabbit Monoclonal SynGAP antibody. Suitable for ICC/IF, WB and reacts with Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SYNGAP1.

View Alternative Names

KIAA1938, SYNGAP1, Ras/Rap GTPase-activating protein SynGAP, Neuronal RasGAP, Synaptic Ras GTPase-activating protein 1, Synaptic Ras-GAP 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SYNGAP1 antibody [HL2292] (AB317190)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SYNGAP1 antibody [HL2292] (AB317190)

ab317190 detects SYNGAP1 protein at cytoplasm by immunofluorescent analysis. Sample : DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green : SYNGAP1 stained by ab317190 diluted at 1 : 250. Red : Tau a axon marker stained by Tau antibody [GT287]diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-SYNGAP1 antibody [HL2292] (AB317190)
  • WB

Supplier Data

Western blot - Anti-SYNGAP1 antibody [HL2292] (AB317190)

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE and the membrane was blotted with ab317190 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SYNGAP1 antibody [HL2292] (ab317190) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2292

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SYNGAP1.

Q96PV0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SynGAP also known as Synaptic Ras GTPase-activating protein 1 has a molecular weight of about 138 kDa. It predominantly expresses in the brain especially in the post-synaptic density of neurons. SynGAP acts mechanically by regulating Ras and Rap GTPase signaling through its GTPase-activating protein (GAP) domain. This protein modulates synaptic strength and plasticity by influencing the activity of these GTPases which are pivotal for intracellular signaling.
Biological function summary

SynGAP influences synaptic function and plasticity. It forms part of the NMDA receptor complex linking it to various synaptic signaling pathways. SynGAP modulates excitatory synaptic transmission by controlling the levels of Ras and Rap GTPases which impact the phosphorylation of AMPA receptors. Through these activities it maintains proper synaptic strength and structural integrity essential for learning and memory processes.

Pathways

SynGAP interfaces with signaling networks critical for neuronal function such as the MAPK/ERK and PI3K/Akt pathways. In these pathways SynGAP regulates Ras-related protein kinase activity influencing downstream effects on gene expression and cellular survival. The protein's interaction with other partners like the PSD-95 and other MAGUK family proteins highlights its central role in controlling synaptic signaling cascades.

Researchers link SynGAP to neurodevelopmental disorders like intellectual disability and autism spectrum disorders. Mutations or disruptions in SynGAP levels correlate with altered synaptic function and contribute to these conditions. SynGAP also interacts with proteins such as SHANK3 which connects to synaptic abnormalities observed in these disorders. Understanding its role in such conditions can provide insights into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. Exhibits dual GTPase-activating specificity for Ras and Rap. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits (By similarity).
See full target information SYNGAP1
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Product promise

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