Rabbit Recombinant Monoclonal Synip antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Images
![Western blot - Anti-Synip antibody [EP302Y] (AB40674), expandable thumbnail](https://content.abcam.com/products/images/synip-antibody-ep302y-ab40674--western-blot-img437479.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Synip antibody [EP302Y] (AB40674)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (AB40674), expandable thumbnail](https://content.abcam.com/products/images/synip-antibody-ep302y-ab40674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437480.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (AB40674)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (AB40674), expandable thumbnail](https://content.abcam.com/products/images/synip-antibody-ep302y-ab40674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437481.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (AB40674)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (AB40674), expandable thumbnail](https://content.abcam.com/products/images/synip-antibody-ep302y-ab40674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437482.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (AB40674)")
Publications
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- Clinical cancer research : an official journal of 23:3442-34522017STXBP4 Drives Tumor Growth and Is Associated with Poor Prognosis through PDGF Receptor Signaling in Lung Squamous Cell Carcinoma.
- Cancer science 100:813-202009Crosstalk between PTEN/Akt2 and TGFbeta signaling involving EGF receptor down-regulation during the tumor promotion process from the early stage in a rat two-stage hepatocarcinogenesis model.Applications:IHC-PReactive species:RatEriko Taniai,Masaomi Kawai,Yasuaki Dewa,Jihei Nishimura,Tomoaki Harada,Yukie Saegusa,Sayaka Matsumoto,Miwa Takahashi,Kunitoshi Mitsumori,Makoto ShibutaniPubMed 19309364
- Biochemical and biophysical research communication 356:102-62007Synip phosphorylation is required for insulin-stimulated Glut4 translocation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuichi Okada et. al.PubMed 17336927
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | IP | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/3500 | Notes - |
Species Rat | Dilution info 1/3500 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Species Mouse | Dilution info 1/5000 | Notes - |
Species Rat | Dilution info 1/5000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose (By similarity).
Alternative names
Syntaxin-binding protein 4, Syntaxin 4-interacting protein, STX4-interacting protein, Synip, STXBP4
Recommended products
Rabbit Recombinant Monoclonal Synip antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP302Y
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is specific for Synip. It is predicted to detect the isoform 2 based an sequence analysis.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product has switched from a hybridoma to recombinant production method on 13th September 2024.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synip also known as Syntaxin-binding protein 4 is a protein with a molecular mass of approximately 78 kDa. This protein modulates intracellular trafficking by binding to syntaxin 4 an important component in vesicle transport. Synip is expressed in various tissues with notable expression in adipose tissue muscle and pancreatic beta cells. Its mechanical role involves managing the accessibility of syntaxin 4 impacting the docking and fusion of vesicles with target membranes.
- Biological function summary
Synip plays a role in exocytosis a process critical for insulin secretion in pancreatic beta cells. Synip forms a complex with other proteins including SNAP-23 and Munc18c which together regulate vesicle docking and fusion. This function is essential for maintaining glucose homeostasis in the body by ensuring proper release of insulin.
- Pathways
Synip interacts with proteins involved in the insulin signaling pathway such as GLUT4 which is responsible for glucose uptake in muscle and adipose tissue. It also plays a role in the SNARE complex formation a critical step in vesicle-mediated transport. Through these interactions Synip contributes to the regulation of glucose metabolism and energy balance in the body.
- Associated diseases and disorders
Synip's function is linked to diabetes mellitus particularly type 2 diabetes due to its role in insulin secretion and glucose homeostasis. Dysregulation or mutations in Synip can impair insulin release leading to insulin resistance. Furthermore Synip has connections with proteins like Syntaxin 4 within the context of metabolic syndrome a condition characterized by increased risk of cardiovascular disease and diabetes due to metabolic imbalances.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Synip antibody [EP302Y] (ab40674), expandable thumbnail](https://content.abcam.com/products/images/synip-antibody-ep302y-ab40674--western-blot-img437479.jpg "Western blot - Anti-Synip antibody [EP302Y] (ab40674)")
Western blot - Anti-Synip antibody [EP302Y] (ab40674)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Synip antibody [EP302Y] (ab40674) at 1/10000 dilution
Lane 1: Human skeletal muscle lysate at 15 µg
Lane 2: Mouse skeletal muscle lysate at 15 µg
Lane 3: Rat skeletal muscle lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Observed band size: 68 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674), expandable thumbnail](https://content.abcam.com/products/images/synip-antibody-ep302y-ab40674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437480.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Synip with ab40674 at 1/1000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674), expandable thumbnail](https://content.abcam.com/products/images/synip-antibody-ep302y-ab40674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437481.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Synip with ab40674 at 1/3500 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674), expandable thumbnail](https://content.abcam.com/products/images/synip-antibody-ep302y-ab40674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437482.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synip antibody [EP302Y] (ab40674)
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Synip with ab40674 at 1/1000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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