Rabbit Polyclonal SYNJ2BP antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SYNJ2BP aa 1 to C-terminus.
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Publications
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- Science (New York, N.Y.) 369:2020The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael J McKenna,Sue Im Sim,Alban Ordureau,Lianjie Wei,J Wade Harper,Sichen Shao,Eunyong ParkPubMed 32973005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SYNJ2BP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P57105
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Function
Regulates endocytosis of activin type 2 receptor kinases through the Ral/RALBP1-dependent pathway and may be involved in suppression of activin-induced signal transduction.
Alternative names
OMP25, SYNJ2BP, Synaptojanin-2-binding protein, Mitochondrial outer membrane protein 25
Recommended products
Rabbit Polyclonal SYNJ2BP antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SYNJ2BP aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SYNJ2BP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P57105
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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10 product images
Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (ab224217)
PFA-fixed, Triton X-100 permeabilized mouse parietal association cortex cells stained for SYNJ2BP (green) using ab224217 at 2 μg/ml in ICC/IF. Shows strong positivity in synapses.
Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (ab224217)
PFA-fixed, Triton X-100 permeabilized mouse hipocampus cells stained for SYNJ2BP (green) using ab224217 at 2 μg/ml in ICC/IF. Shows strong positivity in synapses,
Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (ab224217)
PFA-fixed, Triton X-100 permeabilized mouse globus pallidus cells stained for SYNJ2BP (green) using ab224217 at 2 μg/ml in ICC/IF. Shows weak positivity in synapses and neuronal processes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (ab224217)
ab224217 (1:500 dilution) staining SYNJ2BP of human paraffin-embedded testis tissue by immunohistochemcial analysis shows moderate granular cytoplasmic positivity in seminiferous duct cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (ab224217)
ab224217 (1:500 dilution) staining SYNJ2BP of human paraffin-embedded duodenum tissue by immunohistochemcial analysis shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (ab224217)
ab224217 (1:500 dilution) staining SYNJ2BP of human paraffin-embedded kidney tissue by immunohistochemcial analysis shows moderate granular cytoplasmic positivity in distal tubules cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (ab224217)
ab224217 (1:500 dilution) staining SYNJ2BP of human paraffin-embedded prostate tissue by immunohistochemcial analysis shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (ab224217)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SYNJ2BP (green) using ab224217 at 4 μg/ml in ICC/IF.
Western blot - Anti-SYNJ2BP antibody - N-terminal (ab224217)
All lanes: Western blot - Anti-SYNJ2BP antibody - N-terminal (ab224217) at 1/250 dilution
All lanes: RT4 (human urinary bladder cancer cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 16 kDa
Western blot - Anti-SYNJ2BP antibody - N-terminal (ab224217)
All lanes: Western blot - Anti-SYNJ2BP antibody - N-terminal (ab224217) at 1/250 dilution
All lanes: Mouse cerebral cortex tissue lysate
Developed using the ECL technique.
Predicted band size: 16 kDa
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