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AB224217

Anti-SYNJ2BP antibody - N-terminal

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(2 Publications)

Rabbit Polyclonal SYNJ2BP antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SYNJ2BP aa 1 to C-terminus.

View Alternative Names

OMP25, SYNJ2BP, Synaptojanin-2-binding protein, Mitochondrial outer membrane protein 25

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (AB224217)

ab224217 (1 : 500 dilution) staining SYNJ2BP of human paraffin-embedded duodenum tissue by immunohistochemcial analysis shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (AB224217)

ab224217 (1 : 500 dilution) staining SYNJ2BP of human paraffin-embedded prostate tissue by immunohistochemcial analysis shows moderate to strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (AB224217)

ab224217 (1 : 500 dilution) staining SYNJ2BP of human paraffin-embedded kidney tissue by immunohistochemcial analysis shows moderate granular cytoplasmic positivity in distal tubules cells.

Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (AB224217)

PFA-fixed, Triton X-100 permeabilized mouse parietal association cortex cells stained for SYNJ2BP (green) using ab224217 at 2 μg/ml in ICC/IF. Shows strong positivity in synapses.

Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (AB224217)

PFA-fixed, Triton X-100 permeabilized mouse globus pallidus cells stained for SYNJ2BP (green) using ab224217 at 2 μg/ml in ICC/IF. Shows weak positivity in synapses and neuronal processes.

Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (AB224217)

PFA-fixed, Triton X-100 permeabilized mouse hipocampus cells stained for SYNJ2BP (green) using ab224217 at 2 μg/ml in ICC/IF. Shows strong positivity in synapses,

Western blot - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • WB

Supplier Data

Western blot - Anti-SYNJ2BP antibody - N-terminal (AB224217)

All lanes:

Western blot - Anti-SYNJ2BP antibody - N-terminal (ab224217) at 1/250 dilution

All lanes:

Mouse cerebral cortex tissue lysate

Predicted band size: 16 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYNJ2BP antibody - N-terminal (AB224217)

ab224217 (1 : 500 dilution) staining SYNJ2BP of human paraffin-embedded testis tissue by immunohistochemcial analysis shows moderate granular cytoplasmic positivity in seminiferous duct cells.

Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SYNJ2BP antibody - N-terminal (AB224217)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SYNJ2BP (green) using ab224217 at 4 μg/ml in ICC/IF.

Western blot - Anti-SYNJ2BP antibody - N-terminal (AB224217)
  • WB

Supplier Data

Western blot - Anti-SYNJ2BP antibody - N-terminal (AB224217)

All lanes:

Western blot - Anti-SYNJ2BP antibody - N-terminal (ab224217) at 1/250 dilution

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 16 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SYNJ2BP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P57105

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates endocytosis of activin type 2 receptor kinases through the Ral/RALBP1-dependent pathway and may be involved in suppression of activin-induced signal transduction.
See full target information SYNJ2BP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neural transmission (Vienna, Austria : 1996) : PubMed40886229

2025

The effect of recombinant botulinum neurotoxin A on neuropathic pain in the spared nerve injury mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Rasmus E Hammer,Akinyemi A Omoniyi,Mette Richner,Stephane Lezmi,Christian B Vaegter,Mikhail Kalinichev,Pall Karlsson,Jens R Nyengaard

Science (New York, N.Y.) 369: PubMed32973005

2020

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Sue Im Sim,Alban Ordureau,Lianjie Wei,J Wade Harper,Sichen Shao,Eunyong Park
View all publications
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Product promise

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