AB217373

Anti-Synphilin 1 antibody

Name: Anti-Synphilin 1 antibody (ab217373) | Abcam
Brand: Abcam Limited
SKU: AB217373
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Synphilin 1 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Synphilin-1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Synphilin-1, Sph1, Alpha-synuclein-interacting protein, SNCAIP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (AB217373)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (AB217373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat brain tissue labeling Synphilin 1 with ab217373 at a 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-Synphilin 1 antibody (AB217373)

All lanes:

Western blot - Anti-Synphilin 1 antibody (ab217373) at 1/200 dilution

All lanes:

Rat brain whole cell lysate

Secondary

All lanes:

Goat Anti-Goat Anti-Rabbit IgG (H+L) HRP at 1/3000 dilution

Predicted band size: 100 kDa

Observed band size: 100 kDa,60kDa kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Synphilin-1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6H5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synphilin-1 also known as SNCAIP is a protein that plays a role in cellular mechanisms. It has a molecular mass of approximately 90 kDa. Synphilin-1 is expressed in various tissues with high levels seen in the brain particularly within neurons. This protein undergoes interactions with other cellular components and participates in the regulation of protein aggregation which is critical in maintaining cellular homeostasis.

Biological function summary

Synphilin-1 associates with alpha-synuclein to form complexes that influence neuronal function. It participates in the modulation of synaptic activity and neuronal signaling. The protein functions as a scaffold supporting various signaling molecules and influencing their activity. Synphilin-1's role in these processes highlights its importance in maintaining neural cell health and function.

Pathways

Synphilin-1 influences the ubiquitin-proteasome system that is responsible for protein degradation and turnover. This involvement links Synphilin-1 to the degradation of misfolded proteins a function that is shared with ubiquitin ligases. Another pathway of interest is the endoplasmic reticulum-associated degradation (ERAD) pathway where Synphilin-1 mediates the removal of aberrant proteins. Synphilin-1’s association with the ubiquitin-proteasome pathway and its interactions with related proteins like parkin provide insight into its regulatory functions.

Synphilin-1 exhibits a significant connection to neurodegenerative diseases such as Parkinson's disease. Its interaction with alpha-synuclein an important player in this disorder highlights its involvement in disease pathogenesis. Additionally Synphilin-1 has been linked to Alzheimer's disease where its aggregation-modulating properties may influence disease progression. These relationships make Synphilin-1 a target of interest for therapeutic research in the context of neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.

See full target information Synphilin-1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401634 PubMed38888507

2024

T Cell-Derived Apoptotic Extracellular Vesicles Hydrolyze cGAMP to Alleviate Radiation Enteritis via Surface Enzyme ENPP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhou,Lili Bao,Shengkai Gong,Geng Dou,Zihan Li,Zhengyan Wang,Lu Yu,Feng Ding,Huan Liu,Xiayun Li,Siying Liu,Xiaoshan Yang,Shiyu Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synphilin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (AB217373)

Western blot - Anti-Synphilin 1 antibody (AB217373)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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