AB6179

Anti-Synphilin 1 antibody

BOND RX™ Validated
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(3 Publications)

Rabbit Polyclonal Synphilin 1 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Synphilin-1 aa 900-950 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Synphilin-1, Sph1, Alpha-synuclein-interacting protein, SNCAIP

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (AB6179)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (AB6179)

IHC image of ab6179 staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Synphilin-1 aa 900-950 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6H5

Specificity

Immunohistochemical analysis of human brain indicates a high level of specificity for this antiserum. Cross reactivity: This antibody is known to react with rat and human. Cross reactivity with other species has not been tested yet.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

This antibody stains Lewy bodies in Dorsal raphe nucleus of Parkinson's diseased human brain.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

pH: 7 - 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synphilin-1 also known as SNCAIP is a protein that plays a role in cellular mechanisms. It has a molecular mass of approximately 90 kDa. Synphilin-1 is expressed in various tissues with high levels seen in the brain particularly within neurons. This protein undergoes interactions with other cellular components and participates in the regulation of protein aggregation which is critical in maintaining cellular homeostasis.

Biological function summary

Synphilin-1 associates with alpha-synuclein to form complexes that influence neuronal function. It participates in the modulation of synaptic activity and neuronal signaling. The protein functions as a scaffold supporting various signaling molecules and influencing their activity. Synphilin-1's role in these processes highlights its importance in maintaining neural cell health and function.

Pathways

Synphilin-1 influences the ubiquitin-proteasome system that is responsible for protein degradation and turnover. This involvement links Synphilin-1 to the degradation of misfolded proteins a function that is shared with ubiquitin ligases. Another pathway of interest is the endoplasmic reticulum-associated degradation (ERAD) pathway where Synphilin-1 mediates the removal of aberrant proteins. Synphilin-1’s association with the ubiquitin-proteasome pathway and its interactions with related proteins like parkin provide insight into its regulatory functions.

Synphilin-1 exhibits a significant connection to neurodegenerative diseases such as Parkinson's disease. Its interaction with alpha-synuclein an important player in this disorder highlights its involvement in disease pathogenesis. Additionally Synphilin-1 has been linked to Alzheimer's disease where its aggregation-modulating properties may influence disease progression. These relationships make Synphilin-1 a target of interest for therapeutic research in the context of neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.

See full target information Synphilin-1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Life sciences 221:35-46 PubMed30735733

2019

Panaxadiol inhibits synaptic dysfunction in Alzheimer's disease and targets the Fyn protein in APP/PS1 mice and APP-SH-SY5Y cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xicai Liang,Yingjia Yao,Ying Lin,Liang Kong,Honghe Xiao,Yue Shi,Jingxian Yang

Brain research. Developmental brain research 149:131-42 PubMed15063093

2004

Parkin expression in the developing mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Kati Kühn,Xin-Ran Zhu,Hermann Lübbert,Christine C Stichel

BMC neuroscience 2:11 PubMed11591219

2001

Deletion of the alpha-synuclein locus in a subpopulation of C57BL/6J inbred mice.

Applications

Unspecified application

Species

Unspecified reactive species

C G Specht,R Schoepfer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synphilin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (AB6179)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise