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AB220746

Anti-Syntaxin 12/Syntaxin-13 antibody

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(1 Publication)

Rabbit Polyclonal Syntaxin 12/Syntaxin-13 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Syntaxin-12 aa 100-200.

View Alternative Names

Syntaxin-12, STX12

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 12/Syntaxin-13 antibody (AB220746)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 12/Syntaxin-13 antibody (AB220746)

Immunofluorescent analysis of PFA-fixed/Triton X-100 permeabilized HeLa cells labeling Syntaxin 12/Syntaxin-13 with ab220746 at 4 μg/ml (green).

Western blot - Anti-Syntaxin 12/Syntaxin-13 antibody (AB220746)
  • WB

Supplier Data

Western blot - Anti-Syntaxin 12/Syntaxin-13 antibody (AB220746)

All lanes:

Western blot - Anti-Syntaxin 12/Syntaxin-13 antibody (ab220746) at 1/500 dilution

Lane 1:

RT-4 cell line lysate

Lane 2:

U-251 MG cell line lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Syntaxin-12 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q86Y82

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 12 also known as Syntaxin-13 is a member of the SNARE family of proteins. This protein is essential for vesicular transport mediating the fusion of vesicles with target membranes. Syntaxin 12 has a molecular mass of approximately 35 kDa and is predominantly expressed in human tissues with high presence in the brain. It plays an important role in intracellular trafficking processes influencing the localization and transport of cargo molecules within cells.
Biological function summary

Syntaxin 12 serves in the assembly of SNARE complexes which are important for vesicle docking and membrane fusion events. It particularly associates with other SNARE proteins such as VAMPs and SNAPs to form a complex that drives the merging of vesicles with target membranes. This function is vital in processes such as neurotransmitter release and endocytic recycling where precise coordination of vesicular transport is necessary for cellular and neural functions.

Pathways

Syntaxin 12 actively participates in key intracellular trafficking pathways. It is significantly involved in endocytic recycling and phagocytosis pathways ensuring the proper routing and recycling of receptors and other membrane components. In these pathways Syntaxin 12 interacts with proteins such as SNX27 and small GTPases like Rab proteins which modulate vesicular transport activities. Its function in these pathways highlights its importance for maintaining cellular homeostasis and proper cell communication.

Syntaxin 12 has been linked with neurodegenerative diseases particularly Alzheimer's disease. Abnormal expression or functioning of this protein can disrupt synaptic vesicle trafficking leading to impaired neurotransmission and neural damage. Additionally Syntaxin 12 connections with other proteins such as amyloid precursor protein (APP) suggest its involvement in the pathogenic pathways leading to the accumulation of neurotoxic proteins. Understanding these interactions provides insight into the molecular basis of such neurodegenerative conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNARE promoting fusion of transport vesicles with target membranes. Together with SNARE STX6, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endocytic recycling pathway. Through complex formation with GRIP1, GRIA2 and NSG1 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting.
See full target information Syntaxin-12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 16:6573-6581 PubMed30405797

2018

Therapeutic effects of lenvatinib in combination with rAd-p53 for the treatment of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Renzhi Yu,Minghuan Wang,Xiuli Zhu,Zhe Sun,Aiying Jiang,Huixin Yao
View all publications
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Product promise

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