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AB134945

Anti-Syntaxin 16 antibody [EPR9156]

5

(4 Reviews)

|

(4 Publications)

Rabbit Recombinant Monoclonal Syntaxin 16 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Syntaxin-16, Syn16, STX16

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (AB134945)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (AB134945)

Immunofluorescence staining of HepG2 cells, labelling Syntaxin 16 using ab134945 at a 1/100 dilution (red). Cell nuclei are shown in blue.

Western blot - Anti-Syntaxin 16 antibody [EPR9156] (AB134945)
  • WB

Unknown

Western blot - Anti-Syntaxin 16 antibody [EPR9156] (AB134945)

All lanes:

Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9156

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 16 also known as STX16 is a member of the syntaxin family that has a central role in vesicular trafficking. This protein weighs approximately 37 kDa and is expressed in various tissues with notable presence in the brain and kidney. Syntaxin 16 is an integral membrane protein located mainly in the Golgi apparatus. It interacts with other SNARE proteins to mediate the fusion of vesicles with target membranes facilitating the movement of proteins and lipids within cells.
Biological function summary

Syntaxin 16 is involved in the proper distribution of proteins across cellular compartments. It is a component of the SNARE complex which is essential for membrane fusion events. Syntaxin 16 plays a significant role in endosomal trafficking where it helps in sorting and recycling of membrane proteins. The protein's function ensures that nutrients and signaling molecules are correctly transported and that cellular homeostasis is maintained.

Pathways

Syntaxin 16 is involved in essential intracellular trafficking pathways. One such pathway is the endosomal-lysosomal pathway regulating the recycling and degradation of cellular components. It also plays a part in the transport between the trans-Golgi network and endosomes where it works closely with proteins like VAMP4 and Vti1a. These interactions ensure the directional flow of vesicles and the maintenance of cellular organization.

Syntaxin 16 shows relevance to conditions affecting neurological and renal systems. Syntaxin 16 has been implicated in some neurodegenerative diseases suggesting disruptions in vesicular trafficking may contribute to pathogenesis. Furthermore altered expression or function of Syntaxin 16 affects renal function possibly connecting with proteins like VAMP2 which can influence kidney-related disorders. Understanding Syntaxin 16's role offers valuable insights into the cellular mechanisms underlying these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
See full target information Syntaxin-16
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Traffic (Copenhagen, Denmark) 26:e70003 PubMed40100055

2025

Acute GARP Depletion Disrupts Vesicle Transport, Leading to Severe Defects in Sorting, Secretion and O-Glycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Amrita Khakurel,Irina Pokrovskaya,Walter S Aragon-Ramirez,Vladimir V Lupashin

Frontiers in cell and developmental biology 10:1033501 PubMed36467416

2022

Pleiotropic effects of Syntaxin16 identified by gene editing in cultured adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shaun K Bremner,Woroud S Al Shammari,Roderick S Milligan,Brian D Hudson,Calum Sutherland,Nia J Bryant,Gwyn W Gould

Cells 10: PubMed33572370

2021

Retrograde and Anterograde Transport of Lat-Vesicles during the Immunological Synapse Formation: Defining the Finely-Tuned Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Juan José Saez,Stephanie Dogniaux,Massiullah Shafaq-Zadah,Ludger Johannes,Claire Hivroz,Andrés Ernesto Zucchetti

The Journal of experimental medicine 215:1245-1265 PubMed29440364

2018

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Marie Carpier,Andres E Zucchetti,Laurence Bataille,Stéphanie Dogniaux,Massiullah Shafaq-Zadah,Sabine Bardin,Marco Lucchino,Mathieu Maurin,Leonel D Joannas,Joao Gamelas Magalhaes,Ludger Johannes,Thierry Galli,Bruno Goud,Claire Hivroz
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com