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AB238922

Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Syntaxin 16 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Syntaxin-16, Syn16, STX16

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (AB238922)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (AB238922)

Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung adenocarcinoma tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134945).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (AB238922)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (AB238922)

Immunofluorescence staining of HepG2 cells, labelling Syntaxin 16 using ab134945 at a 1/100 dilution (red). Cell nuclei are shown in blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134945).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9156

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab238922 is the carrier-free version of ab134945.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 16 also known as STX16 is a member of the syntaxin family that has a central role in vesicular trafficking. This protein weighs approximately 37 kDa and is expressed in various tissues with notable presence in the brain and kidney. Syntaxin 16 is an integral membrane protein located mainly in the Golgi apparatus. It interacts with other SNARE proteins to mediate the fusion of vesicles with target membranes facilitating the movement of proteins and lipids within cells.
Biological function summary

Syntaxin 16 is involved in the proper distribution of proteins across cellular compartments. It is a component of the SNARE complex which is essential for membrane fusion events. Syntaxin 16 plays a significant role in endosomal trafficking where it helps in sorting and recycling of membrane proteins. The protein's function ensures that nutrients and signaling molecules are correctly transported and that cellular homeostasis is maintained.

Pathways

Syntaxin 16 is involved in essential intracellular trafficking pathways. One such pathway is the endosomal-lysosomal pathway regulating the recycling and degradation of cellular components. It also plays a part in the transport between the trans-Golgi network and endosomes where it works closely with proteins like VAMP4 and Vti1a. These interactions ensure the directional flow of vesicles and the maintenance of cellular organization.

Syntaxin 16 shows relevance to conditions affecting neurological and renal systems. Syntaxin 16 has been implicated in some neurodegenerative diseases suggesting disruptions in vesicular trafficking may contribute to pathogenesis. Furthermore altered expression or function of Syntaxin 16 affects renal function possibly connecting with proteins like VAMP2 which can influence kidney-related disorders. Understanding Syntaxin 16's role offers valuable insights into the cellular mechanisms underlying these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
See full target information Syntaxin-16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan
View all publications
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Product promise

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