Rabbit Recombinant Monoclonal Syntaxin 16 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (AB238922), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-16-antibody-epr9156-bsa-and-azide-free-ab238922--immunocytochemistry-immunofluorescence-img26420.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (AB238922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (AB238922), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-16-antibody-epr9156-bsa-and-azide-free-ab238922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136786.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (AB238922)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Predicted | Predicted |
| Rat | Not recommended | Not recommended | Not recommended | Predicted | Predicted |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
Alternative names
Syntaxin-16, Syn16, STX16
Recommended products
Rabbit Recombinant Monoclonal Syntaxin 16 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9156
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238922 is the carrier-free version of Anti-Syntaxin 16 antibody [EPR9156] ab134945.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syntaxin 16 also known as STX16 is a member of the syntaxin family that has a central role in vesicular trafficking. This protein weighs approximately 37 kDa and is expressed in various tissues with notable presence in the brain and kidney. Syntaxin 16 is an integral membrane protein located mainly in the Golgi apparatus. It interacts with other SNARE proteins to mediate the fusion of vesicles with target membranes facilitating the movement of proteins and lipids within cells.
- Biological function summary
Syntaxin 16 is involved in the proper distribution of proteins across cellular compartments. It is a component of the SNARE complex which is essential for membrane fusion events. Syntaxin 16 plays a significant role in endosomal trafficking where it helps in sorting and recycling of membrane proteins. The protein's function ensures that nutrients and signaling molecules are correctly transported and that cellular homeostasis is maintained.
- Pathways
Syntaxin 16 is involved in essential intracellular trafficking pathways. One such pathway is the endosomal-lysosomal pathway regulating the recycling and degradation of cellular components. It also plays a part in the transport between the trans-Golgi network and endosomes where it works closely with proteins like VAMP4 and Vti1a. These interactions ensure the directional flow of vesicles and the maintenance of cellular organization.
- Associated diseases and disorders
Syntaxin 16 shows relevance to conditions affecting neurological and renal systems. Syntaxin 16 has been implicated in some neurodegenerative diseases suggesting disruptions in vesicular trafficking may contribute to pathogenesis. Furthermore altered expression or function of Syntaxin 16 affects renal function possibly connecting with proteins like VAMP2 which can influence kidney-related disorders. Understanding Syntaxin 16's role offers valuable insights into the cellular mechanisms underlying these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (ab238922), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-16-antibody-epr9156-bsa-and-azide-free-ab238922--immunocytochemistry-immunofluorescence-img26420.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (ab238922)")
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (ab238922)
Immunofluorescence staining of HepG2 cells, labelling Syntaxin 16 using Anti-Syntaxin 16 antibody [EPR9156] ab134945 at a 1/100 dilution (red). Cell nuclei are shown in blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Syntaxin 16 antibody [EPR9156] ab134945).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (ab238922), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-16-antibody-epr9156-bsa-and-azide-free-ab238922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136786.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (ab238922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (ab238922)
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung adenocarcinoma tissue, labelling Syntaxin 16 using Anti-Syntaxin 16 antibody [EPR9156] ab134945 at a 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Syntaxin 16 antibody [EPR9156] ab134945).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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