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AB249256

Anti-Syntaxin 18 antibody [EPR8939] - BSA and Azide free

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Rabbit Recombinant Monoclonal Syntaxin 18 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human samples.

View Alternative Names

GIG9, STX18, Syntaxin-18, Cell growth-inhibiting gene 9 protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 18 antibody [EPR8939] - BSA and Azide free (AB249256)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 18 antibody [EPR8939] - BSA and Azide free (AB249256)

This data was developed using ab156017, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Syntaxin 18 with ab156017 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 18 antibody [EPR8939] - BSA and Azide free (AB249256)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 18 antibody [EPR8939] - BSA and Azide free (AB249256)

This data was developed using ab156017, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Syntaxin 18 with ab156017 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Syntaxin 18 antibody [EPR8939] - BSA and Azide free (AB249256)
  • WB

Unknown

Western blot - Anti-Syntaxin 18 antibody [EPR8939] - BSA and Azide free (AB249256)

This data was developed using ab156017, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Syntaxin 18 antibody [EPR8939] (<a href='/en-us/products/primary-antibodies/syntaxin-18-antibody-epr8939-ab156017'>ab156017</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Fetal heart tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8939

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab249256 is the carrier-free version of ab156017.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 18 also known as STX18 is a membrane protein that plays an important role in intracellular protein transport. This protein has an approximate molecular mass of 34.5 kDa and primarily resides in the endoplasmic reticulum (ER). Syntaxin 18 helps mediate vesicle trafficking facilitating the movement of different molecules between the ER and the Golgi apparatus. It acts as a target-specific SNARE protein which stands for "soluble N-ethylmaleimide-sensitive factor attachment protein receptor." SNARE proteins are known for their role in fusion processes of transport vesicles to their target membranes.
Biological function summary

Syntaxin 18 is involved in maintaining cellular homeostasis. It functions as part of a larger complex often referred to as the Syntaxin 18 complex which also includes other proteins such as SNARE partners Use1 BNIP1 and Sec22b. This protein complex is essential in the post-translational transport of proteins from the ER to the Golgi. Syntaxin 18 ensures precise vesicle targeting and fusion which is important for proper cell function and protein maturation processes within the secretory pathway.

Pathways

Syntaxin 18 integrates into the secretory pathway where it coordinates with other proteins like VAMP7 and SNAP29 to facilitate vesicle transport. This pathway is vital for the regulation of protein and lipid shipping within cells. Syntaxin 18 also plays an integral role in the ER-to-Golgi transport vesicles which are important in protein secretion and autophagy. As Syntaxin 18 is linked to proteins involved in vesicular trafficking it is critical in ensuring that cellular components are distributed efficiently within the cell.

Syntaxin 18 has associations with neurodegenerative disorders such as Alzheimer's disease due to its role in protein traffic dysregulation. Abnormal expression or mutations in Syntaxin 18 can disrupt vesicular transport leading to cellular stress and pathological aggregates that relate to these diseases. Furthermore Syntaxin 18 interacts with proteins like VAMP7 which also impacts processes implicated in certain autoimmune diseases. Understanding these interactions provides insights into how impaired Syntaxin 18 function contributes to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Syntaxin that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.
See full target information STX18
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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