Rabbit Polyclonal Syntaxin 1a antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 18 publications.
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- Neurochemical research 48:791-8032022Munc18-1 Contributes to Hippocampal Injury in Septic Rats Through Regulation of Syntanxin1A and Synaptophysin and Glutamate Levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFajuan Tang,Lin Chen,Hu Gao,Yupeng Lei,Linli Pan,Dongqiong Xiao,Xihong LiPubMed 36335177
- Biomedicines 9:2021Neuromodulation Induced by Sitagliptin: A New Strategy for Treating Diabetic Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHugo Ramos,Patricia Bogdanov,David Sabater,Jordi Huerta,Marta Valeri,Cristina Hernández,Rafael SimóPubMed 34944588
- PLoS biology 19:e30013232021Synaptotagmin-7-mediated activation of spontaneous NMDAR currents is disrupted in bipolar disorder susceptibility variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiu-Wen Wang,Ying-Han Wang,Bing Wang,Yun Chen,Si-Yao Lu,Jun YaoPubMed 34228711
- ACS chemical neuroscience 12:2529-25412021Wide-Ranging Effects on the Brain Proteome in a Transgenic Mouse Model of Alzheimer's Disease Following Treatment with a Brain-Targeting Somatostatin Peptide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFadi Rofo,Friederike A Sandbaumhüter,Aikaterini Chourlia,Nicole G Metzendorf,Jamie I Morrison,Stina Syvänen,Per E Andrén,Erik T Jansson,Greta HultqvistPubMed 34170117
- International journal of molecular sciences 22:2021High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlaria Zuliani,Chiara Lanzillotta,Antonella Tramutola,Eugenio Barone,Marzia Perluigi,Serena Rinaldo,Alessio Paone,Francesca Cutruzzolà,Francesco Bellanti,Matteo Spinelli,Francesca Natale,Salvatore Fusco,Claudio Grassi,Fabio Di DomenicoPubMed 33916835
- British journal of pharmacology 177:5658-56762020Glycogen synthase kinase-3 inhibition rescues sex-dependent contextual fear memory deficit in human immunodeficiency virus-1 transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShamsudheen Moidunny,Michael A Benneyworth,David J Titus,Eleonore Beurel,Udhghatri Kolli,Joyce Meints,Richa Jalodia,Sundaram Ramakrishnan,Coleen M Atkins,Sabita RoyPubMed 33080056
- PloS one 15:e02341472020Enhanced phosphorylation of PERK in primary cultured neurons as an autonomous neuronal response to prion infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMisaki Tanaka,Takeshi Yamasaki,Rie Hasebe,Akio Suzuki,Motohiro HoriuchiPubMed 32479530
- Neuroscience letters 709:1343642019Synaptic structure and alterations in the hippocampus in neonatal rats exposed to lipopolysaccharide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanfen Lin et. al.PubMed 31288048
- eLife 8:2019Endocytic recycling and vesicular transport systems mediate transcytosis of across cell monolayer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Li et. al.PubMed 31012847
- Molecular brain 12:112019Baicalin regulates the dopamine system to control the core symptoms of ADHD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongyi Zhou et. al.PubMed 30736828
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Mouse | Tested | Tested | Expected |
| Rat | Expected | Expected | Tested |
| Cow | Predicted | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Macaque monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Macaque monkey, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Macaque monkey, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed:17301173, PubMed:18167541, PubMed:20484665, PubMed:22411134, PubMed:28813412, PubMed:7901002). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane (PubMed:14665625, PubMed:16888141, PubMed:19571812). STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion (PubMed:14665625, PubMed:16888141, PubMed:19571812). Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (By similarity). Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (By similarity).
Alternative names
Sap, Syntaxin-1A, Neuron-specific antigen HPC-1, Synaptotagmin-associated 35 kDa protein, P35A
Recommended products
Rabbit Polyclonal Syntaxin 1a antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syntaxin 1a also known as STX1A is an essential protein in cellular mechanisms involved in neurotransmitter release. This protein weighing approximately 33 kDa plays a direct role in synaptic vesicle fusion through its function as a member of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein Receptor) complex. Syntaxin 1a is highly expressed in the brain especially in areas like the hippocampus and cerebral cortex where it facilitates rapid neurotransmission important for communication between neurons.
- Biological function summary
The involvement of Syntaxin 1a in synaptic transmission is significant. It forms a complex with other proteins such as SNAP-25 and VAMP enabling the docking and fusion of synaptic vesicles with the presynaptic membrane. This action results in the release of neurotransmitters into the synaptic cleft which then bind to receptors on the postsynaptic neuron to propagate neural signals. Syntaxin 1a ensures efficient synaptic activity and is indispensable for maintaining synaptic plasticity which is critical for learning and memory.
- Pathways
Syntaxin 1a is pivotal in the regulation of neurotransmitter release within the synaptic vesicle cycle pathway. This pathway includes the assembly and disassembly of the SNARE complex which are processes vital for neurotransmitter exocytosis. Syntaxin 1a engages closely with proteins like synaptotagmin acting as calcium sensors and Munc18 which helps in initiating SNARE assembly. These interactions highlight the careful orchestration of vesicle trafficking and fusion events necessary for effective synaptic communication.
- Associated diseases and disorders
Dysfunctions in Syntaxin 1a are linked to neurological conditions such as epilepsy and autism spectrum disorders. Alterations or mutations in Syntaxin 1a can lead to improper synaptic vesicle fusion and disrupted neurotransmitter release which are characteristics of these disorders. Additionally Syntaxin 1a interacts with proteins like Munc18-1 whose mutations are associated with similar synaptic transmission deficiencies further implicating the involvement of Syntaxin 1a in these pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunoprecipitation - Anti-Syntaxin 1a antibody (ab41453)
Syntaxin 1a was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Syntaxin 1a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41453.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 33kDa, non specific band - 25kDa: We are unsure as to the identity of this extra band; Syntaxin 1aAll lanes: Immunoprecipitation - Anti-Syntaxin 1a antibody (ab41453)
Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 1a antibody (ab41453)
ab41453 staining Syntaxin 1a in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab41453 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Syntaxin 1a antibody (ab41453)
All lanes: Western blot - Anti-Syntaxin 1a antibody (ab41453) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Brain (Mouse) Mouse Tissue Lysate at 10 µg
Lane 3: Brain (Rat) Rat Tissue Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 1a antibody (ab41453)
ICC/IF image of ab41453 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41453, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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