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AB249569

Anti-Syntaxin 1a antibody [EPR11072(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Syntaxin 1a antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

STX1, STX1A, Syntaxin-1A, Neuron-specific antigen HPC-1

3 Images
Flow Cytometry (Intracellular) - Anti-Syntaxin 1a antibody [EPR11072(B)] - BSA and Azide free (AB249569)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Syntaxin 1a antibody [EPR11072(B)] - BSA and Azide free (AB249569)

This data was developed using ab170890, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling Syntaxin 1a with ab170890 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11072(B)] - BSA and Azide free (AB249569)
  • IP

Supplier Data

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11072(B)] - BSA and Azide free (AB249569)

This data was developed using ab170890, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from human fetal brain lysate using ab170890.

Lane 1 Primary : ab170890, 1/10000 dilution
Sample : Immunoprecipitation pellet from Human fetal brain lysate

Predicted band 33 kDa

All lanes:

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11072(B)] (<a href='/en-us/products/primary-antibodies/syntaxin-1a-antibody-epr11072b-ab170890'>ab170890</a>)

Predicted band size: 33 kDa

false

Western blot - Anti-Syntaxin 1a antibody [EPR11072(B)] - BSA and Azide free (AB249569)
  • WB

Supplier Data

Western blot - Anti-Syntaxin 1a antibody [EPR11072(B)] - BSA and Azide free (AB249569)

This data was developed using ab170890, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Syntaxin 1a antibody [EPR11072(B)] (<a href='/en-us/products/primary-antibodies/syntaxin-1a-antibody-epr11072b-ab170890'>ab170890</a>) at 1/10000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

Human cerebellum lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11072(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249569 is the carrier-free version of ab170890.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 1a also known as STX1A is an essential protein in cellular mechanisms involved in neurotransmitter release. This protein weighing approximately 33 kDa plays a direct role in synaptic vesicle fusion through its function as a member of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein Receptor) complex. Syntaxin 1a is highly expressed in the brain especially in areas like the hippocampus and cerebral cortex where it facilitates rapid neurotransmission important for communication between neurons.
Biological function summary

The involvement of Syntaxin 1a in synaptic transmission is significant. It forms a complex with other proteins such as SNAP-25 and VAMP enabling the docking and fusion of synaptic vesicles with the presynaptic membrane. This action results in the release of neurotransmitters into the synaptic cleft which then bind to receptors on the postsynaptic neuron to propagate neural signals. Syntaxin 1a ensures efficient synaptic activity and is indispensable for maintaining synaptic plasticity which is critical for learning and memory.

Pathways

Syntaxin 1a is pivotal in the regulation of neurotransmitter release within the synaptic vesicle cycle pathway. This pathway includes the assembly and disassembly of the SNARE complex which are processes vital for neurotransmitter exocytosis. Syntaxin 1a engages closely with proteins like synaptotagmin acting as calcium sensors and Munc18 which helps in initiating SNARE assembly. These interactions highlight the careful orchestration of vesicle trafficking and fusion events necessary for effective synaptic communication.

Dysfunctions in Syntaxin 1a are linked to neurological conditions such as epilepsy and autism spectrum disorders. Alterations or mutations in Syntaxin 1a can lead to improper synaptic vesicle fusion and disrupted neurotransmitter release which are characteristics of these disorders. Additionally Syntaxin 1a interacts with proteins like Munc18-1 whose mutations are associated with similar synaptic transmission deficiencies further implicating the involvement of Syntaxin 1a in these pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed : 26635000). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (PubMed : 23091057). Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (By similarity).
See full target information STX1A
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Product promise

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