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AB170889

Anti-Syntaxin 1a antibody [EPR11073(B)]

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(2 Publications)

Rabbit Recombinant Monoclonal Syntaxin 1a antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

STX1, STX1A, Syntaxin-1A, Neuron-specific antigen HPC-1

2 Images
Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11073(B)] (AB170889)
  • IP

Supplier Data

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11073(B)] (AB170889)

Western blot analysis on immunoprecipitation pellet from human fetal brain lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170889 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Lane 1 Primary : ab170889, 1/1000 dilution
Sample : Immunoprecipitated Human fetal brain lysate
Secondary : HRP-conjugated anti-rabbit IgG

Lane 2 Primary : ab170889, 1/1000 dilution
Sample : 1X PBS (negative control)
Secondary : HRP-conjugated anti-rabbit IgG

Predicted band 33 kDa

All lanes:

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)

Predicted band size: 33 kDa

false

Western blot - Anti-Syntaxin 1a antibody [EPR11073(B)] (AB170889)
  • WB

Supplier Data

Western blot - Anti-Syntaxin 1a antibody [EPR11073(B)] (AB170889)

All lanes:

Western blot - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Fetal brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

  • Carrier free

    Anti-Syntaxin 1a antibody [EPR11073(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11073(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 1a also known as STX1A is an essential protein in cellular mechanisms involved in neurotransmitter release. This protein weighing approximately 33 kDa plays a direct role in synaptic vesicle fusion through its function as a member of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein Receptor) complex. Syntaxin 1a is highly expressed in the brain especially in areas like the hippocampus and cerebral cortex where it facilitates rapid neurotransmission important for communication between neurons.
Biological function summary

The involvement of Syntaxin 1a in synaptic transmission is significant. It forms a complex with other proteins such as SNAP-25 and VAMP enabling the docking and fusion of synaptic vesicles with the presynaptic membrane. This action results in the release of neurotransmitters into the synaptic cleft which then bind to receptors on the postsynaptic neuron to propagate neural signals. Syntaxin 1a ensures efficient synaptic activity and is indispensable for maintaining synaptic plasticity which is critical for learning and memory.

Pathways

Syntaxin 1a is pivotal in the regulation of neurotransmitter release within the synaptic vesicle cycle pathway. This pathway includes the assembly and disassembly of the SNARE complex which are processes vital for neurotransmitter exocytosis. Syntaxin 1a engages closely with proteins like synaptotagmin acting as calcium sensors and Munc18 which helps in initiating SNARE assembly. These interactions highlight the careful orchestration of vesicle trafficking and fusion events necessary for effective synaptic communication.

Dysfunctions in Syntaxin 1a are linked to neurological conditions such as epilepsy and autism spectrum disorders. Alterations or mutations in Syntaxin 1a can lead to improper synaptic vesicle fusion and disrupted neurotransmitter release which are characteristics of these disorders. Additionally Syntaxin 1a interacts with proteins like Munc18-1 whose mutations are associated with similar synaptic transmission deficiencies further implicating the involvement of Syntaxin 1a in these pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed : 26635000). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (PubMed : 23091057). Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (By similarity).
See full target information STX1A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurochemistry 158:182-196 PubMed33894004

2021

RNA editing-mediated regulation of calcium-dependent activator protein for secretion (CAPS1) localization and its impact on synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla M Shumate,Sadik T Tas,Ege T Kavalali,Ronald B Emeson

Frontiers in neurology 8:281 PubMed28713327

2017

IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Gao,Fei Li,Ziwei Zhou,Xin Xu,Yingang Wu,Shuai Zhou,Dongpei Yin,Dongdong Sun,Jianhua Xiong,Rongcai Jiang,Jianning Zhang
View all publications
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