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Rabbit Recombinant Monoclonal Syntaxin 1a antibody. Suitable for IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] (AB272736), expandable thumbnail
  • Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (AB272736), expandable thumbnail
  • Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (AB272736), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Syntaxin 1a antibody [EPR23457-15] (AB272736), expandable thumbnail
  • Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (AB272736), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-FrFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Expected
Expected
Expected
Tested
Mouse
Tested
Tested
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Tested
Tested
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed:26635000). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (PubMed:23091057). Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (By similarity).

Alternative names

STX1, STX1A, Syntaxin-1A, Neuron-specific antigen HPC-1

Recommended products

Rabbit Recombinant Monoclonal Syntaxin 1a antibody. Suitable for IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23457-15
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Syntaxin 1a also known as STX1A is an essential protein in cellular mechanisms involved in neurotransmitter release. This protein weighing approximately 33 kDa plays a direct role in synaptic vesicle fusion through its function as a member of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein Receptor) complex. Syntaxin 1a is highly expressed in the brain especially in areas like the hippocampus and cerebral cortex where it facilitates rapid neurotransmission important for communication between neurons.

Biological function summary

The involvement of Syntaxin 1a in synaptic transmission is significant. It forms a complex with other proteins such as SNAP-25 and VAMP enabling the docking and fusion of synaptic vesicles with the presynaptic membrane. This action results in the release of neurotransmitters into the synaptic cleft which then bind to receptors on the postsynaptic neuron to propagate neural signals. Syntaxin 1a ensures efficient synaptic activity and is indispensable for maintaining synaptic plasticity which is critical for learning and memory.

Pathways

Syntaxin 1a is pivotal in the regulation of neurotransmitter release within the synaptic vesicle cycle pathway. This pathway includes the assembly and disassembly of the SNARE complex which are processes vital for neurotransmitter exocytosis. Syntaxin 1a engages closely with proteins like synaptotagmin acting as calcium sensors and Munc18 which helps in initiating SNARE assembly. These interactions highlight the careful orchestration of vesicle trafficking and fusion events necessary for effective synaptic communication.

Associated diseases and disorders

Dysfunctions in Syntaxin 1a are linked to neurological conditions such as epilepsy and autism spectrum disorders. Alterations or mutations in Syntaxin 1a can lead to improper synaptic vesicle fusion and disrupted neurotransmitter release which are characteristics of these disorders. Additionally Syntaxin 1a interacts with proteins like Munc18-1 whose mutations are associated with similar synaptic transmission deficiencies further implicating the involvement of Syntaxin 1a in these pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736), expandable thumbnail

    Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

    Syntaxin 1a was immunoprecipitated from 0.35 mg human brain tissue lysate 10μg with ab272736 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272736 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human brain tissue lysate 10μg.

    Lane 2: ab272736 IP in human brain tissue lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab272736 in human brain tissue lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    All lanes: Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

  • Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736), expandable thumbnail

    Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what have been described in literature (PMID: 10644452).

    Negative control: Human liver (PMID: 10644452).

    Exposure time: 3.25 seconds.

    All lanes: Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736) at 1/2000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

  • Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736), expandable thumbnail

    Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    All lanes: Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736) at 1/2000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

  • Flow Cytometry (Intracellular) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Syntaxin 1a with ab272736 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736), expandable thumbnail

    Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes Lane 2: 3.25 seconds Lane 3: 15 seconds.

    All lanes: Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/2000, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on rat cerebrum (PMID: 10644452).

    The section was incubated with ab272736 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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