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AB314152

Anti-Syntaxin 1A + Syntaxin 1B antibody [EPR25899-4] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Syntaxin antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
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Sandwich ELISA - Anti-Syntaxin 1A + Syntaxin 1B antibody [EPR25899-4] - BSA and Azide free (Capture) (AB314152)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Syntaxin 1A + Syntaxin 1B antibody [EPR25899-4] - BSA and Azide free (Capture) (AB314152)

Sandwich ELISA of ab314151 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25899-4

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab314152 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Syntaxin 1B. The recommended pair for sandwich ELISA is:

Capture: ab314152, Syntaxin 1A + Syntaxin 1B Capture Antibody (unconjugated)

Detector: ab314153, Syntaxin 1B Detector Antibody (unconjugated)

The reference range value is 0.78-50 ng/ml.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 1A and Syntaxin 1B are integral proteins in the nervous system functioning in the vesicle docking and fusion process. These proteins are part of the SNARE (Soluble NSF Attachment Protein Receptor) family and facilitate neurotransmitter release at synapses. Syntaxin 1A has an approximate molecular weight of 33 kDa and Syntaxin 1B has a similar mass. Both proteins are expressed in brain tissues particularly in neurons where they are abundant at presynaptic membranes.
Biological function summary

The role of Syntaxin 1A and Syntaxin 1B is to mediate synaptic vesicle exocytosis. They function as part of the SNARE complex which also includes SNAP-25 and VAMP/synaptobrevin. This complex formation is essential for the proper alignment and fusion of vesicles with the presynaptic membrane facilitating efficient neurotransmitter release. This process is important for neuronal communication and synaptic plasticity.

Pathways

Syntaxin 1A and Syntaxin 1B are central components in neurotransmission and synaptic signaling pathways. These proteins interact with other components such as synaptotagmin which acts as a calcium sensor in neurotransmitter release. The proteins' actions are integrated into the broader calcium signaling pathway which regulates neurotransmitter release in response to synaptic activity.

Syntaxin 1A and Syntaxin 1B have connections to neurological conditions like epilepsy and autism spectrum disorders. In epilepsy abnormalities in Syntaxin 1 function may disrupt normal neurotransmitter release leading to seizures. In autism altered synaptic function involving these proteins might contribute to the disorder's pathophysiology. Another protein related to Syntaxin 1 in these conditions is Munc18-1 which regulates Syntaxin activity by stabilizing the inactive form of Syntaxin in the SNARE complex.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm (By similarity).
See full target information STX1B

Additional targets

STX1A

Alternative Names

STX1B1, STX1B2, STX1B, Syntaxin-1B, Syntaxin-1B1, Syntaxin-1B2, STX1, STX1A, Syntaxin-1A, Neuron-specific antigen HPC-1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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