JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314153

Anti-Syntaxin 1B antibody [EPR25899-7] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Syntaxin antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
1 Images
Sandwich ELISA - Anti-Syntaxin 1B antibody [EPR25899-7] - BSA and Azide free (Detector) (AB314153)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Syntaxin 1B antibody [EPR25899-7] - BSA and Azide free (Detector) (AB314153)

Sandwich ELISA of ab314151 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25899-7

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB316901

Human Syntaxin 1B ELISA Kit

SimpleStep

Sandwich ELISA - Human Syntaxin 1B ELISA Kit (AB316901)

  • 0
  • 0
Sample Prep & Detection Kits

AB269900

PE/Atto 594 Conjugation Kit - Lightning-Link®

Schematic Diagram - PE/Atto 594 Conjugation Kit - Lightning-Link® (AB269900)

  • 0
  • 0
Sample Prep & Detection Kits

AB270703

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (AB270703)

  • 0
  • 0
Primary Antibodies

AB242612

Anti-Angiopoietin 2/ANG2 antibody [EPR17258-118] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Angiopoietin 2/ANG2 antibody [EPR17258-118] - BSA and Azide free (Capture) (AB242612)

  • 0
  • 0
Primary Antibodies

AB259752

Anti-RAB10 antibody [EPR13242] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (Detector) (AB259752)

  • 0
  • 0
Primary Antibodies

AB242736

Anti-Prolactin/PRL antibody [EPR18018-5] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Prolactin/PRL antibody [EPR18018-5] - BSA and Azide free (Detector) (AB242736)

  • 0
  • 0
Primary Antibodies

AB242782

Anti-MMP8 antibody [EPR17824-39] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-MMP8 antibody [EPR17824-39] - BSA and Azide free (Detector) (AB242782)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab314153 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Syntaxin 1B. The recommended pair for sandwich ELISA is:

Capture: ab314152, Syntaxin 1A + Syntaxin 1B Capture Antibody (unconjugated)

Detector: ab314153, Syntaxin 1B Detector Antibody (unconjugated)

The reference range value is 0.78-50 ng/ml.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 1B often abbreviated as STX1B is an essential SNARE protein with a mass of approximately 33 kDa. This protein is mainly expressed in neurons and tissues with neural characteristics indicating its prominent role in the nervous system. Mechanically Syntaxin 1B facilitates the docking and fusion of synaptic vesicles with the presynaptic membrane. Through this mechanism it aids in the release of neurotransmitters which is fundamental for synaptic transmission and communication between neurons.
Biological function summary

This protein functions as a core component of the SNARE complex comprising Syntaxin 1B SNAP-25 and VAMP2. The formation of this complex brings the synaptic vesicle and presynaptic membrane into close proximity creating the condition for vesicle fusion. Syntaxin 1B plays a significant role in synaptic efficiency and plasticity impacting processes such as learning and memory.

Pathways

Syntaxin 1B is integral to the neurotransmitter release pathway and exocytosis pathways. It closely interacts with proteins such as SNAP-25 and VAMP2 both of which are critical for the proper execution of synaptic vesicle release. Within these pathways Syntaxin 1B assists in the regulated release of chemical messengers ultimately influencing neuronal communication and overall neural network activity.

Mutations or dysfunctions in Syntaxin 1B have links to neurological conditions such as epilepsy and intellectual disability. Research indicates that abnormalities in Syntaxin 1B might disrupt synaptic transmission potentially through altered interactions with proteins like SNAP-25. These disruptions can lead to erratic neuronal firing patterns or inadequate synaptic connectivity contributing to the pathophysiology observed in these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm (By similarity).
See full target information STX1B

Alternative Names

STX1B1, STX1B2, STX1B, Syntaxin-1B, Syntaxin-1B1, Syntaxin-1B2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com