Rabbit Polyclonal Syntaxin 2/STX2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Rat Stx2 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Frontiers in cell and developmental biology 9:6159732021STX2 Promotes Trophoblast Growth, Migration, and Invasion Through Activation of the PI3K-AKT Pathway in Preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Li,Xian-Li Sun,Chun-Ling Ma,Chao Li,Ying Zhan,Wen-Ting Li,Can Li,Yi-Hao WangPubMed 34295885
- Molecular medicine reports 18:1015-10242018Munc13‑4 mediates human neutrophil elastase‑induced airway mucin5AC hypersecretion by interacting with syntaxin2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Xu et. al.PubMed 29767240
- PloS one 11:e01583862016HIV and HCV Co-Culture Promotes Profibrogenic Gene Expression through an Epimorphin-Mediated ERK Signaling Pathway in Hepatic Stellate Cells.
- Biology of reproduction 85:721-322011Deficiency in the omega-3 fatty acid pathway results in failure of acrosome biogenesis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuel Roqueta-Rivera,Timothy L Abbott,Mayandi Sivaguru,Rex A Hess,Manabu T NakamuraPubMed 21653892
- Nature cell biology 9:1286-932007SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity.
- American journal of physiology. Gastrointestinal a 292:G1249-622007Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling.
- Archives of andrology 52:347-532006Localization of SNAREs, NSF and Caveolin 1 in human spermatozoa: relationship with seminal parameters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA P M Sousa,C S S Gomes-Santos,J Ramalho-SantosPubMed 16873134
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Stx2 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P50279
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes human and 1/1000-1/2000 for other species. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
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Target data
Function
Essential for epithelial morphogenesis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
Alternative names
EPIM, STX2A, STX2B, STX2C, STX2, Syntaxin-2, Epimorphin
Recommended products
Rabbit Polyclonal Syntaxin 2/STX2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Rat Stx2 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Stx2 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P50279
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was purified on a peptide bound immunoaffinity column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syntaxin 2 (STX2) also known as epimorphin plays an important role in the process of vesicle fusion in cells. This protein is part of the t-SNARE family and has a molecular mass of approximately 33 kDa. STX2 is expressed in various tissues including the brain lungs and kidneys indicating its widespread involvement in cellular processes throughout the body.
- Biological function summary
Syntaxin 2 mediates intracellular membrane fusion events. It functions as a component of the SNARE complex which facilitates vesicle targeting and fusion with cell membranes. This activity is necessary for secretory pathways and endocytic processes ensuring proper transport and release of cellular materials. In addition STX2 takes part in regulating insulin exocytosis highlighting its role in maintaining metabolic balance.
- Pathways
The protein is central to the vesicular transport and secretory pathways. It interacts with other SNARE proteins like SNAP-25 and VAMP2 forming a complex that drives membrane fusion. Syntaxin 2 also has a connection with the insulin signaling pathway highlighting its contribution to glucose homeostasis and energy metabolism. These interactions show its participation in critical cellular communication and transport pathways.
- Associated diseases and disorders
Syntaxin 2 is linked to certain metabolic and neurological conditions. Its involvement in insulin secretion suggests a connection to type 2 diabetes where dysregulation can affect insulin release. Additionally altered expression or function of STX2 has been examined in neurodegenerative diseases such as Alzheimer's disease. Disruptions involving proteins like APP in conjunction with STX2 might contribute to the development of such disorders indicating STX2's potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Syntaxin 2/STX2 antibody (ab12369)
All lanes: Western blot - Anti-Syntaxin 2/STX2 antibody (ab12369) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: Human kidney tissue lysate - total protein (ab30203) at 10 µg
Lane 4: Human skin tissue lysate - total protein (ab30166) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33.3 kDa, 74 kDa
Exposure time: 4min
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 2/STX2 antibody (ab12369)
ICC/IF image of ab12369 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12369, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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