Rabbit Polyclonal Syntaxin 2/STX2 antibody. Suitable for WB, IHC-P and reacts with Pig, Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human STX2 aa 1 to C-terminus.
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Publications
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- Nature communications 11:35942020CircGRIA1 shows an age-related increase in male macaque brain and regulates synaptic plasticity and synaptogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiyu Xu et. al.PubMed 32681011
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human STX2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P32856
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Pig | Tested | Expected |
| Recombinant fragment - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Recombinant fragment - Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Essential for epithelial morphogenesis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
Alternative names
EPIM, STX2A, STX2B, STX2C, STX2, Syntaxin-2, Epimorphin
Recommended products
Rabbit Polyclonal Syntaxin 2/STX2 antibody. Suitable for WB, IHC-P and reacts with Pig, Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human STX2 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human STX2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P32856
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
ab233275 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syntaxin 2 (STX2) also known as epimorphin plays an important role in the process of vesicle fusion in cells. This protein is part of the t-SNARE family and has a molecular mass of approximately 33 kDa. STX2 is expressed in various tissues including the brain lungs and kidneys indicating its widespread involvement in cellular processes throughout the body.
- Biological function summary
Syntaxin 2 mediates intracellular membrane fusion events. It functions as a component of the SNARE complex which facilitates vesicle targeting and fusion with cell membranes. This activity is necessary for secretory pathways and endocytic processes ensuring proper transport and release of cellular materials. In addition STX2 takes part in regulating insulin exocytosis highlighting its role in maintaining metabolic balance.
- Pathways
The protein is central to the vesicular transport and secretory pathways. It interacts with other SNARE proteins like SNAP-25 and VAMP2 forming a complex that drives membrane fusion. Syntaxin 2 also has a connection with the insulin signaling pathway highlighting its contribution to glucose homeostasis and energy metabolism. These interactions show its participation in critical cellular communication and transport pathways.
- Associated diseases and disorders
Syntaxin 2 is linked to certain metabolic and neurological conditions. Its involvement in insulin secretion suggests a connection to type 2 diabetes where dysregulation can affect insulin release. Additionally altered expression or function of STX2 has been examined in neurodegenerative diseases such as Alzheimer's disease. Disruptions involving proteins like APP in conjunction with STX2 might contribute to the development of such disorders indicating STX2's potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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8 product images
Western blot - Anti-Syntaxin 2/STX2 antibody (ab233275)
All lanes: Western blot - Anti-Syntaxin 2/STX2 antibody (ab233275) at 1 µg/mL
Lane 1: Human serum
Lane 2: Human lung lysate
Lane 3: Pig brain lysate
Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
SecondaryAll lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 2/STX2 antibody (ab233275)
Paraffin-embedded human breast cancer tissue stained for Syntaxin 2/STX2 using ab233275 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 2/STX2 antibody (ab233275)
Paraffin-embedded human glioma tissue stained for Syntaxin 2/STX2 using ab233275 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 2/STX2 antibody (ab233275)
Paraffin-embedded human lung cancer tissue stained for Syntaxin 2/STX2 using ab233275 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 2/STX2 antibody (ab233275)
Paraffin-embedded human kidney tissue stained for Syntaxin 2/STX2 using ab233275 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 2/STX2 antibody (ab233275)
Paraffin-embedded human stomach tissue stained for Syntaxin 2/STX2 using ab233275 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 2/STX2 antibody (ab233275)
Paraffin-embedded human pancreas tissue stained for Syntaxin 2/STX2 using ab233275 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Syntaxin 2/STX2 antibody (ab233275)
All lanes: Western blot - Anti-Syntaxin 2/STX2 antibody (ab233275) at 1 µg
All lanes: Recombinant human Syntaxin 2/STX2 protein
SecondaryAll lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 33 kDa
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