Anti-Syntaxin 3 antibody [EPR8543]

9 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Syntaxin 3 with Purified ab133750 at 1:50 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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  Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  All lanes: Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution

  Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

  Lane 2: Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

  Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

  Lane 4: Rat lung lysates at 20 µg

  Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 33 kDa

  Observed band size: 33 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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  Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  All lanes: Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution

  Lane 1: HepG2 cell lysate at 10 µg

  Lane 2: 293T cell lysate at 10 µg

  Lane 3: BxPC-3 cell lysate at 10 µg

  Lane 4: Raw 264.7 cell lysate at 10 µg

  Lane 5: PC12 cell lysate at 10 µg

  Lane 6: NIH3T3 cell lysate at 10 µg

  Lane 7: Caco-2 cell lysate at 10 µg

  Secondary

  All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

  Predicted band size: 33 kDa

  Observed band size: 33 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Syntaxin 3 with ab133750 at 1/50.
  

  Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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  Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  Immunocytochemical analysis of HepG2 cells labelling Syntaxin 3 with ab133750 at 1/50.

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  OI-RD Scanning - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

  We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
  Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.