Anti-Syntaxin 3 antibody [EPR8543]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

Immunocytochemical analysis of HepG2 cells labelling Syntaxin 3 with ab133750 at 1/50.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Syntaxin 3 with ab133750 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1 : 250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1 : 250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Syntaxin 3 with Purified ab133750 at 1 : 50 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1 : 250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Unknown

Western blot - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

All lanes:

Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Lane 4:

Raw 264.7 cell lysate at 10 µg

Lane 5:

PC12 cell lysate at 10 µg

Lane 6:

NIH3T3 cell lysate at 10 µg

Lane 7:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

• WB

Lab

Western blot - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

All lanes:

Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

Lane 4:

Rat lung lysates at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.