Rabbit Recombinant Monoclonal Syntaxin 3 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (AB248638), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-3-antibody-epr8543-bsa-and-azide-free-ab248638--immunocytochemistry-immunofluorescence-img95070.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (AB248638)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (AB248638), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-3-antibody-epr8543-bsa-and-azide-free-ab248638--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104827.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (AB248638)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (AB248638), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-3-antibody-epr8543-bsa-and-azide-free-ab248638--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83270.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (AB248638)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (AB248638), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-3-antibody-epr8543-bsa-and-azide-free-ab248638--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106017.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (AB248638)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Predicted | Not recommended | Not recommended | Expected | Predicted |
| Mouse | Tested | Not recommended | Not recommended | Expected | Expected |
| Rat | Expected | Not recommended | Not recommended | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Potentially involved in docking of synaptic vesicles at presynaptic active zones. Apical receptor involved in membrane fusion of apical vesicles. Isoform B. Essential for survival of retinal photoreceetors. Isoform 3. Functions as a regulator of gene expression.
Alternative names
STX3A, STX3, Syntaxin-3
Recommended products
Rabbit Recombinant Monoclonal Syntaxin 3 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8543
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.34 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248638 is the carrier-free version of Anti-Syntaxin 3 antibody [EPR8543] ab133750.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syntaxin 3 sometimes known as Stx3 is a protein involved in vesicle trafficking and membrane fusion. It plays a mechanical role by facilitating the fusion of transport vesicles with target membranes an essential step in intracellular transport mechanisms. Syntaxin 3 is a member of the t-SNARE family and has a molecular mass of approximately 33 kDa. This protein expresses mainly in epithelial cells including those in the kidney and lungs as well as certain neuronal tissues indicating its involvement in critical cellular processes in these areas.
- Biological function summary
Syntaxin 3 acts in cellular transport by participating in the trans-Golgi network-to-plasma membrane pathway. This protein is part of the SNARE complex a group of proteins that mediates vesicle fusion. By engaging with other SNARE proteins like SNAP-25 and VAMP Syntaxin 3 helps ensure the precise delivery of vesicle contents to the plasma membrane. Its role is important for maintaining cellular homeostasis by regulating how cells communicate and exchange materials with their environment.
- Pathways
Syntaxin 3 is an integral component of the exocytosis pathway and is involved in the secretory pathway. In exocytosis Syntaxin 3 associates with proteins such as Munc18 and synaptotagmin which help in vesicle docking and fusion. In the secretory pathway Syntaxin 3 is responsible for directing proteins and lipids to their intended membrane sites thereby influencing cellular communication. These interactions guide the flow of proteins within vesicle transport networks and coordinate various signaling events within the cell.
- Associated diseases and disorders
Syntaxin 3 has connections to conditions such as cystic fibrosis and certain types of diabetes. In cystic fibrosis abnormal function of Syntaxin 3 may disrupt normal secretory processes in epithelial cells contributing to enhanced pathology. The protein is associated with the cystic fibrosis transmembrane conductance regulator (CFTR) protein which fails to localize correctly in this disease. In diabetes Syntaxin 3 impacts insulin secretion due to its role in pancreatic beta cells tying it to the disorder through insulin-regulating mechanisms with proteins such as GLUT4.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-3-antibody-epr8543-bsa-and-azide-free-ab248638--immunocytochemistry-immunofluorescence-img95070.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638)")
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638)
Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Syntaxin 3 with Purified Anti-Syntaxin 3 antibody [EPR8543] ab133750 at 1:50 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Syntaxin 3 antibody [EPR8543] ab133750)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-3-antibody-epr8543-bsa-and-azide-free-ab248638--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104827.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Syntaxin 3 with Purified Anti-Syntaxin 3 antibody [EPR8543] ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Syntaxin 3 antibody [EPR8543] ab133750)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-3-antibody-epr8543-bsa-and-azide-free-ab248638--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83270.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Syntaxin 3 with Purified Anti-Syntaxin 3 antibody [EPR8543] ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Syntaxin 3 antibody [EPR8543] ab133750)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638), expandable thumbnail](https://content.abcam.com/products/images/syntaxin-3-antibody-epr8543-bsa-and-azide-free-ab248638--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106017.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free (ab248638)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Syntaxin 3 with Purified Anti-Syntaxin 3 antibody [EPR8543] ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Syntaxin 3 antibody [EPR8543] ab133750)
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