Rabbit Polyclonal Syntaxin 4 antibody. N-terminal. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human STX4 aa 1-50.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human STX4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q12846
Reactivity data
Select an application| IP | |
|---|---|
| Human | Tested |
| Cat | Predicted |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Cynomolgus monkey | Predicted |
| Dog | Predicted |
| Gorilla | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Rhesus monkey | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.
Alternative names
Syntaxin-4, Renal carcinoma antigen NY-REN-31, STX4, STX4A
Recommended products
Rabbit Polyclonal Syntaxin 4 antibody. N-terminal. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human STX4 aa 1-50.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human STX4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q12846
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab226218 was affinity purified using an epitope specific to Syntaxin 4 immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab226218 has not performed satisfactorily when used for WB of Syntaxin 4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Syntaxin 4.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syntaxin 4 also known as STX4 is a member of the soluble NSF attachment protein receptor (SNARE) family. It has a molecular mass of approximately 33 kDa. Syntaxin 4 is integral to membrane fusion processes specifically docking and fusion of vesicles with target membranes. It expresses in a wide range of tissues but high levels are found in skeletal muscle and adipose tissue. Its function is necessary in cellular trafficking systems and exocytosis.
- Biological function summary
Syntaxin 4 facilitates key transport events at the plasma membrane. It forms part of the SNARE complex which is essential for vesicle targeting and fusion. This complex includes other SNARE proteins such as VAMP2 and SNAP23 ensuring that vesicles deliver their content accurately within cells. This function is critical in processes such as insulin-stimulated glucose uptake and neurotransmitter release.
- Pathways
Syntaxin 4 involves itself in the regulation of vesicular transport and insulin signaling. It plays an essential role in the GLUT4 translocation pathway where Syntaxin 4 interacts with proteins like Munc18c which is critical for the regulation of glucose transport in response to insulin. Another important pathway includes its involvement in neurotransmitter release where Syntaxin 4 interfaces with synaptotagmin highlighting its influence within signal transmission processes.
- Associated diseases and disorders
Dysfunctions related to Syntaxin 4 have implications in conditions such as type 2 diabetes and certain neuropathies. In the context of type 2 diabetes impaired Syntaxin 4-mediated uptake of glucose into cells can lead to insulin resistance. Syntaxin 4 also relates to neuromuscular disorders where its malfunction can alter neurotransmitter release. These conditions oftentimes involve Munc18c which stabilizes Syntaxin 4 and modulates its activity within the cell.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunoprecipitation - Anti-Syntaxin 4 antibody - N-terminal (ab226218)
Syntaxin 4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226218 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-Syntaxin 4 antibody at 1 μg/ml.
Lane 1: ab226218 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-Syntaxin 4 antibody - N-terminal (ab226218)
Predicted band size: 34 kDa
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