Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (AB140607)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (AB140607)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (AB140607)

All lanes:

Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607) at 1/1000 dilution

Lane 1:

PC-12 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa

false

• WB

CiteAb

Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (AB140607)

Syntaxin 6 western blot using anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker ab140607. Publication image and figure legend from Seko, Y., Fujimura, T., et al., 2015, Sci Rep, PubMed 26348594.

ab140607 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab140607 please see the product overview.

Identification of the structural modifications present in the secreted form of eIF5A.(a–c) Extracted ion chromatogram of the +3 charge state of the apparent tryptic peptide of residues 68–85 (KYEDICPSTHNMDVPNIK + 80 Da) of eIF-5A (a) and that of the corresponding theoretically sulfated (b) and phosphorylated (c) peptides. The spectrum (a) was extracted from the full mass chromatogram (m/z 350–2000). The lower two theoretical spectra were calculated as the tryptic peptide plus SO3 (b) or HPO3 (c) based on the element composition. (d) The +3 charge state for MS/MS of the apparently sulfated peptide of residues 68–85 (m/z 728.52). (e) Western blot analysis of cytosolic and secreted re-eIF5A for tyrosine sulfation (upper panel) and eIF5A (lower panel) using YSP5-45-36. (f) Effects of hypoxia (60 min)/reoxygenation on the translocation of eIF5A to the trans-Golgi (labeled with syntaxin 6 [lower panel]) and on tyrosine sulfation (upper panel) or on the localization of eIF5A (middle panel) using YSP5-45-36.

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