AB243719

Anti-Syntaxin 6 antibody - Golgi Membrane Marker

Name: Anti-Syntaxin 6 antibody - Golgi Membrane Marker (ab243719) | Abcam
Brand: Abcam Limited
SKU: AB243719
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Syntaxin 6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STX6 aa 50-200.

View Alternative Names

Syntaxin-6, STX6

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (AB243719)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (AB243719)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Syntaxin 6 using ab243719 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (AB243719)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (AB243719)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Syntaxin 6 (green) using ab243719 at 4 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (AB243719)

All lanes:

Western blot - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (ab243719) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line0 cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver

Lane 5:

Human tonsil

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human STX6 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

O43752

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 6 also known as STX6 functions as a SNARE protein involved in vesicle fusion processes. It has a molecular weight of about 31 kDa. You find it mainly in the trans-Golgi network and within endosomal compartments. Syntaxin 6 varies in expression across different tissues but exhibits strong presence in the brain and lung. This protein plays an important role in intracellular trafficking by mediating fusion of transport vesicles with their correct target membranes.

Biological function summary

Syntaxin 6 contributes to intracellular material transport and acts as a component of the SNARE complex which includes other proteins like Vti1a and VAMP4. These SNARE complexes facilitate vesicle docking and fusion important for cargo delivery. In particular Syntaxin 6 aids in routing proteins and lipids from the Golgi apparatus to their destinations supporting normal cellular function and maintenance of cellular organelles.

Pathways

Syntaxin 6 involves itself in endocytosis and the secretory pathway. It interacts with proteins such as syntaxin 16 and Vti1b coordinating vesicular traffic from the Golgi to endosomes and lysosomes. Proper function of these pathways maintains cellular homeostasis and impacts processes like hormone secretion and receptor recycling making Syntaxin 6 integral for efficient cellular communication and transport.

Dysregulation of Syntaxin 6 links to neurodevelopmental disorders and cancer progression. Abnormal Syntaxin 6 expression can affect neurotransmitter release highlighting its relationship with proteins like VAMP2 in neurological disorders. Further alterations in its function impact cellular proliferation contributing to oncogenic processes through interactions with proteins such as SNAP23. Understanding its role in these contexts emphasizes the need for further research to explore therapeutic implications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SNARE promoting movement of transport vesicles to target membranes. Targets endosomes to the trans-Golgi network, and may therefore function in retrograde trafficking. Together with SNARE STX12, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endocytic recycling pathway.

See full target information STX6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of healthcare engineering 2022:9121554 PubMed35494512

2022

Antitumor Activity of lncRNA NBAT-1 via Inhibition of miR-4504 to Target to WWC3 in Oxaliplatin-Resistant Colorectal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Li,Xu Li

Journal of bone oncology 28:100360 PubMed33996428

2021

Hsa_circ_0010220 regulates miR-198/Syntaxin 6 axis to promote osteosarcoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoan Lu,Chuanwen Wang,Xiaolong Lv,Wen Dai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Syntaxin 6 antibody - Golgi Membrane Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (AB243719)

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (AB243719)

Western blot - Anti-Syntaxin 6 antibody - Golgi Membrane Marker (AB243719)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise