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Rabbit Recombinant Multiclonal Syntaxin 6 antibody. Suitable for WB, ICC/IF, IP and reacts with Dog, Human samples. Immunogen corresponding to Synthetic Peptide within Human STX6.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440), expandable thumbnail
  • Immunoprecipitation - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440), expandable thumbnail
  • Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440), expandable thumbnail
  • Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440), expandable thumbnail
  • Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Synthetic Peptide within Human STX6. Database link O43752

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIP
Human
Tested
Tested
Tested
Dog
Tested
Expected
Expected

Tested
Tested

Species
Dog
Dilution info
0.50000-1.00000 µg/mL
Notes

-

Species
Human
Dilution info
0.50000-1.00000 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
2 µg/mL
Notes

-

Expected
Expected

Species
Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

SNARE promoting movement of transport vesicles to target membranes. Targets endosomes to the trans-Golgi network, and may therefore function in retrograde trafficking. Together with SNARE STX12, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endocytic recycling pathway.

Alternative names

Syntaxin-6, STX6

Recommended products

Rabbit Recombinant Multiclonal Syntaxin 6 antibody. Suitable for WB, ICC/IF, IP and reacts with Dog, Human samples. Immunogen corresponding to Synthetic Peptide within Human STX6.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Synthetic Peptide within Human STX6. Database link O43752
Clone number
RP23040114
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Syntaxin 6 also known as STX6 functions as a SNARE protein involved in vesicle fusion processes. It has a molecular weight of about 31 kDa. You find it mainly in the trans-Golgi network and within endosomal compartments. Syntaxin 6 varies in expression across different tissues but exhibits strong presence in the brain and lung. This protein plays an important role in intracellular trafficking by mediating fusion of transport vesicles with their correct target membranes.

Biological function summary

Syntaxin 6 contributes to intracellular material transport and acts as a component of the SNARE complex which includes other proteins like Vti1a and VAMP4. These SNARE complexes facilitate vesicle docking and fusion important for cargo delivery. In particular Syntaxin 6 aids in routing proteins and lipids from the Golgi apparatus to their destinations supporting normal cellular function and maintenance of cellular organelles.

Pathways

Syntaxin 6 involves itself in endocytosis and the secretory pathway. It interacts with proteins such as syntaxin 16 and Vti1b coordinating vesicular traffic from the Golgi to endosomes and lysosomes. Proper function of these pathways maintains cellular homeostasis and impacts processes like hormone secretion and receptor recycling making Syntaxin 6 integral for efficient cellular communication and transport.

Associated diseases and disorders

Dysregulation of Syntaxin 6 links to neurodevelopmental disorders and cancer progression. Abnormal Syntaxin 6 expression can affect neurotransmitter release highlighting its relationship with proteins like VAMP2 in neurological disorders. Further alterations in its function impact cellular proliferation contributing to oncogenic processes through interactions with proteins such as SNAP23. Understanding its role in these contexts emphasizes the need for further research to explore therapeutic implications.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440)

    Syntaxin 6 Immunocytochemistry/ Immunofluorescence staining of MCF7 cells using rabbit Anti-Syntaxin 6 antibody

    Immunofluorescent analysis of MCF-7 cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling Syntaxin-6 with ab313440 at 1 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of Syntaxin-6 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Syntaxin-6. Panel e) represents control cells with no primary Antibody to assess background.

  • Immunoprecipitation - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440), expandable thumbnail

    Immunoprecipitation - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440)

    Syntaxin 6 was immunoprecipitated from SK-N-SH cell extracts with ab313440 at 2 ?g. Western blot was performed from the immunoprecipitate using ab313440. Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution was used as secondary antibody

    All lanes: Immunoprecipitation - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440) at 2 µg/mL

    Lane 1: Input Control

    Lane 2: Isotype Control

    Lane 3: IP Elute

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 29.176 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440), expandable thumbnail

    Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440)

    Syntaxin 6 Western blot staining using rabbit Anti-Syntaxin 6 antibody

    All lanes: Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440)

    Lane 1: SK-N-SH cell lysate at 30 µg

    Lane 2: SH-SY5Y cell lysate at 30 µg

    Lane 3: IMR-32 cell lysate at 30 µg

    Lane 4: HT-29 cell lysate at 30 µg

    Lane 5: Caco-2 cell lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 29.176 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440), expandable thumbnail

    Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440)

    Syntaxin 6 Western blot staining using rabbit Anti-Syntaxin 6 antibody

    All lanes: Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440) at 0.5 µg/mL

    Lane 1: SH-SY5Y cell lysate at 30 µg

    Lane 2: U-87MG cell lysate at 30 µg

    Lane 3: SK-N-SH cell lysate at 30 µg

    Lane 4: MDCK cell lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 29.176 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440), expandable thumbnail

    Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440)

    Syntaxin 6 Western blot staining using rabbit Anti-Syntaxin 6 antibody

    All lanes: Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440) at 1/1000 dilution

    Lane 1: untransfected SH-SY5Y cell lysate

    Lane 2: non-specific scrambled siRNA transfected SH-SY5Y cell lysate

    Lane 3: Syntaxin 6 knockdown SH-SY5Y cell lysate

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Predicted band size: 29.176 kDa

    Observed band size: 30 kDa

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Product protocols

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