AB313440

Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker

Recombinant
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Rabbit Recombinant Multiclonal Syntaxin 6 antibody. Suitable for WB, ICC/IF, IP and reacts with Dog, Human samples. Immunogen corresponding to Synthetic Peptide within Human STX6.

View Alternative Names

Syntaxin-6, STX6

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

Immunofluorescent analysis of MCF-7 cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling Syntaxin-6 with ab313440 at 1 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of Syntaxin-6 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Syntaxin-6. Panel e) represents control cells with no primary Antibody to assess background.

Supplier Data

Immunoprecipitation - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

Syntaxin 6 was immunoprecipitated from SK-N-SH cell extracts with ab313440 at 2 µg. Western blot was performed from the immunoprecipitate using ab313440. Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution was used as secondary antibody

All lanes:

Immunoprecipitation - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440) at 2 µg/mL

Lanes 1 and 3:

SK-N-SH cell extracts

Lane 2:

SK-N-SH cell extracts with Isotype Control

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 29-176 kDa

Observed band size: 30 kDa

true

Supplier Data

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

All lanes:

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440) at 0.5 µg/mL

Lane 1:

SH-SY5Y cell lysate at 30 µg

Lane 2:

U-87MG cell lysate at 30 µg

Lane 3:

SK-N-SH cell lysate at 30 µg

Lane 4:

MDCK cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 29.176 kDa

Observed band size: 30 kDa

true

Supplier Data

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

All lanes:

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440)

Lane 1:

SK-N-SH cell lysate at 30 µg

Lane 2:

SH-SY5Y cell lysate at 30 µg

Lane 3:

IMR-32 cell lysate at 30 µg

Lane 4:

HT-29 cell lysate at 30 µg

Lane 5:

Caco-2 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 29.176 kDa

Observed band size: 30 kDa

true

Supplier Data

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

All lanes:

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (ab313440) at 1/1000 dilution

Lane 1:

untransfected SH-SY5Y cell lysate

Lane 2:

non-specific scrambled siRNA transfected SH-SY5Y cell lysate

Lane 3:

Syntaxin 6 knockdown SH-SY5Y cell lysate

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 29.176 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040114

Isotype

IgG

Carrier free

Reacts with

Dog, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Synthetic Peptide within Human STX6.

O43752

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2 µg/mL", "IP-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 6 also known as STX6 functions as a SNARE protein involved in vesicle fusion processes. It has a molecular weight of about 31 kDa. You find it mainly in the trans-Golgi network and within endosomal compartments. Syntaxin 6 varies in expression across different tissues but exhibits strong presence in the brain and lung. This protein plays an important role in intracellular trafficking by mediating fusion of transport vesicles with their correct target membranes.

Biological function summary

Syntaxin 6 contributes to intracellular material transport and acts as a component of the SNARE complex which includes other proteins like Vti1a and VAMP4. These SNARE complexes facilitate vesicle docking and fusion important for cargo delivery. In particular Syntaxin 6 aids in routing proteins and lipids from the Golgi apparatus to their destinations supporting normal cellular function and maintenance of cellular organelles.

Pathways

Syntaxin 6 involves itself in endocytosis and the secretory pathway. It interacts with proteins such as syntaxin 16 and Vti1b coordinating vesicular traffic from the Golgi to endosomes and lysosomes. Proper function of these pathways maintains cellular homeostasis and impacts processes like hormone secretion and receptor recycling making Syntaxin 6 integral for efficient cellular communication and transport.

Dysregulation of Syntaxin 6 links to neurodevelopmental disorders and cancer progression. Abnormal Syntaxin 6 expression can affect neurotransmitter release highlighting its relationship with proteins like VAMP2 in neurological disorders. Further alterations in its function impact cellular proliferation contributing to oncogenic processes through interactions with proteins such as SNAP23. Understanding its role in these contexts emphasizes the need for further research to explore therapeutic implications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

SNARE promoting movement of transport vesicles to target membranes. Targets endosomes to the trans-Golgi network, and may therefore function in retrograde trafficking. Together with SNARE STX12, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endocytic recycling pathway.

See full target information STX6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

Immunoprecipitation - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

Western blot - Anti-Syntaxin 6 antibody [RP23040114] - Golgi Membrane Marker (AB313440)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise