AB224223

Anti-Syntaxin 7 antibody

Name: Anti-Syntaxin 7 antibody (ab224223) | Abcam
Brand: Abcam Limited
SKU: AB224223
Price: 645 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Syntaxin 7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human STX7 aa 1-200.

View Alternative Names

Syntaxin-7, STX7

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

Paraffin-embedded human pancreas tissue stained for Syntaxin 7 using ab224223 at 1/200 dilution in immunohistochemical analysis. Shows low positivity in exocrine glandular cells as expected.

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 7 antibody (AB224223)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 7 antibody (AB224223)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Syntaxin 7 (green) using ab224223 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

Paraffin-embedded human lymph node tissue stained for Syntaxin 7 using ab224223 at 1/200 dilution in immunohistochemical analysis. Shows strong membranous positivity in germinal center cells.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

Paraffin-embedded human cerebral cortex tissue stained for Syntaxin 7 using ab224223 at 1/200 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in neurons.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

Paraffin-embedded human kidney tissue stained for Syntaxin 7 using ab224223 at 1/200 dilution in immunohistochemical analysis. Shows strong membranous positivity in tubule cells.

Supplier Data

Western blot - Anti-Syntaxin 7 antibody (AB224223)

All lanes:

Western blot - Anti-Syntaxin 7 antibody (ab224223) at 1/250 dilution

All lanes:

Daudi (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 29 kDa

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Supplier Data

Western blot - Anti-Syntaxin 7 antibody (AB224223)

All lanes:

Western blot - Anti-Syntaxin 7 antibody (ab224223) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 29 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human STX7 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O15400

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 7 also known as STX7 is a member of the SNARE protein family important for vesicle fusion processes. With a molecular mass of approximately 29 kDa Syntaxin 7 plays a significant role in the intracellular trafficking system by mediating membrane fusion events. It is expressed predominantly in many tissues including the brain and spleen where it contributes to diverse cellular functions. As a member of the target membrane SNARE proteins Syntaxin 7 partners with other SNARE proteins to facilitate endolysosomal transport.

Biological function summary

Syntaxin 7 mediates membrane fusion events essential for endosome-lysosome fusion. It forms a complex with other SNARE proteins like Vti1b Syntaxin 8 and VAMP8. This complex orchestrates the accurate trafficking and degradation of macromolecules within cells. Syntaxin 7's presence proves essential in ensuring molecules are delivered to lysosomes for degradation and it participates in recycling cellular components. This role helps maintain cellular homeostasis by regulating the turnover of cellular substances.

Pathways

Syntaxin 7 plays vital roles in processes related to vesicle transport and autophagy. It contributes significantly to the endocytic pathway an important pathway responsible for importing molecules into cells and directing them to lysosomes for breakdown. Syntaxin 7 is also involved in the autophagic-lysosomal pathway working in conjunction with proteins like Syntaxin 8 to facilitate autophagosome and lysosome fusion. Its coordination in these pathways highlights the importance of Syntaxin 7’s role in maintaining cellular health and balance.

Syntaxin 7 has been linked to conditions related to defective lysosomal storage and neurodegenerative diseases. In Alzheimer’s disease for instance impaired endosomal and lysosomal functions are typical and dysfunction of proteins like Syntaxin 7 can exacerbate these issues. Similarly abnormal expression of Syntaxin 7 could contribute to neuronal loss observed in Alzheimer's disease. Its interaction with other SNARE proteins in this context suggests that targeting Syntaxin 7 and its complex could offer therapeutic potential for addressing related cellular dysfunctions in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.

See full target information STX7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 633:932-940 PubMed39232161

2024

CDK5-cyclin B1 regulates mitotic fidelity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Feng Zheng,Aniruddha Sarkar,Humphrey Lotana,Aleem Syed,Huy Nguyen,Richard G Ivey,Jacob J Kennedy,Jeffrey R Whiteaker,Bartłomiej Tomasik,Kaimeng Huang,Feng Li,Alan D D'Andrea,Amanda G Paulovich,Kavita Shah,Alexander Spektor,Dipanjan Chowdhury

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Syntaxin 7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 7 antibody (AB224223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 7 antibody (AB224223)

Western blot - Anti-Syntaxin 7 antibody (AB224223)

Western blot - Anti-Syntaxin 7 antibody (AB224223)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise